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CALY Antibody (aa141‑190) LS‑C135003
CALY antibody LS-C135003 is an unconjugated rabbit polyclonal antibody to CALY from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C135003-50
50 µg
Unavailable

Popular CALY Products

Antibody
Species: Human, Mouse, Rat
Applications: Western blot
Western blot analysis of CALY expression in A549 (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
Species: Human, Mouse, Rat
Applications: Western blot
Anti-CALY Antibody at 1:2000 dilution + human brain lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-CALY Antibody at 1:2000 dilution + human brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 23 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human, Mouse
Applications: Western blot

Product Description

CALY antibody LS-C135003 is an unconjugated rabbit polyclonal antibody to CALY from human, mouse, rat and other species. Validated for WB.
About CALY
Interacts with clathrin light chain A and stimulates clathrin self-assembly and clathrin-mediated endocytosis. Q9NYX4 NM_015722 NP_056537.1

CALY Antibody, Calcyon protein Antibody, CALCYON Antibody, NSG3 Antibody, RP11-122K13.5 Antibody, DRD1IP Antibody


Specifications

Target
Rat CALY
Host
Rabbit
Reactivity
Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
CALY antibody was raised against synthetic peptide located between aa141-190 of rat Caly (P58821, NP_620270). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Rabbit, Guinea pig (100%); Bovine, Pig (85%); Elephant (84%); Hamster, Bat (83%).
Blocking Peptide
LS-E17113 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135003. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa141-190
Specificity
Rat CALY
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

CALY antibody LS-C135003 Western blot of Rat Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CALY antibody LS-C135003 Western blot of Rat Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CALY antibody LS-C135003 Western blot of Rat Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CALY antibody LS-C135003 Western blot of Rat Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CALY antibody LS-C135003 Western blot of Rat Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CALY antibody LS-C135003 Western blot of Rat Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CALY antibody LS-C135003 Western blot of Rat Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CALY antibody LS-C135003 Western blot of Rat Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/18/2018

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