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Anti-BUB1B / BubR1 Antibody (clone 6E3) LS-C114772


Wt. Vol. Conc. Price
- 100 µl - $350
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular BUB1B / BubR1 Antibodies

Anti-BUB1B / BubR1 Antibody (aa1-350, clone 8G1) LS-C2771
Mouse Monoclonal [clone 8G1] (IgG1,k) to Human BUB1B / BubR1
Human, Mouse
Immunofluorescence, Western blot
Immunofluorescence Image
Anti-BUB1B / BubR1 Antibody (clone 2G5) IHC-plus™ LS-B4289
Mouse Monoclonal [clone 2G5] (IgG,k) to Human BUB1B / BubR1
IHC - Paraffin, Western blot, ELISA, Proximity Ligation Assay, RNA interference
Immunohistochemistry Image
Anti-BUB1B / BubR1 Antibody IHC-plus™ LS-B4854
Rabbit Polyclonal to Human BUB1B / BubR1
IHC - Paraffin, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 6E3] (IgG1) to Human BUB1B / BubR1
Human, Monkey, Rat, Dog
Immunofluorescence, Western blot, Flow Cytometry


Human BUB1B / BubR1
Human, Monkey, Rat, Dog (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [6E3]
Protein A/G purified


  • Immunofluorescence (1:100)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

BUB1B / BubR1 antibody was raised against full length human recombinant protein of human BUB1B (NP_001202) produced in HEK293T cell.
Human BUBR1
Concentration: 0.40 mg/ml (Lot dependent)


PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About BUB1B / BubR1

O60566 NM_001211 NP_001202.4

BUB1B Antibody, Bub1A Antibody, BUBR1 Antibody, HBUBR1 Antibody, Protein SSK1 Antibody, SSK1 Antibody, BUB1beta Antibody, MAD3L Antibody

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE.


Anti-BUB1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.

Western blot

Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.

Flow Cytometry

Flow Cytometry
Flow cytometry of Jurkat cells, using anti-BUB1B antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

Flow Cytometry
Flow cytometry of HeLa cells, using anti-BUB1B antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BUB1B antibody, and then analyzed by flow cytometry.

Requested From: United States
Date Requested: 10/27/2016

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