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Anti-BUB1B / BubR1 Antibody (clone 11D7) LS-C114638

Ordering

Wt. Vol. Conc. Price
- 100 µl - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BUB1B / BubR1 Antibodies

Anti-BUB1B / BubR1 Antibody (aa1-350, clone 8G1) LS-C2771
Mouse Monoclonal [clone 8G1] (IgG1,k) to Human BUB1B / BubR1
Human, Mouse
Immunofluorescence, Western blot
Unconjugated
Immunofluorescence Image
Anti-BUB1B / BubR1 Antibody (clone 2G5) IHC-plus™ LS-B4289
Mouse Monoclonal [clone 2G5] (IgG,k) to Human BUB1B / BubR1
Human
IHC - Paraffin, Western blot, ELISA, Proximity Ligation Assay, RNA interference
Unconjugated
Immunohistochemistry Image
Anti-BUB1B / BubR1 Antibody IHC-plus™ LS-B4854
Rabbit Polyclonal to Human BUB1B / BubR1
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 11D7] (IgG1) to Human BUB1B / BubR1
Human, Monkey
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human BUB1B / BubR1
Mouse
Human, Monkey (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [11D7]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC - Paraffin
  • Immunofluorescence (1:100)
  • Western blot

Specificity and Use

BUB1B / BubR1 antibody was raised against protein expressed in 293T cell transfected with human BUB1B expression vector.
Human BUBR1
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About BUB1B / BubR1

O60566 NM_001211 NP_001202.4

BUB1B Antibody, Bub1A Antibody, BUBR1 Antibody, HBUBR1 Antibody, Protein SSK1 Antibody, SSK1 Antibody, BUB1beta Antibody, MAD3L Antibody

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-BUB1B mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Carcinoma of liver tissue using anti-BUB1B mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-BUB1B mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BUB1B mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Immunofluorescence
Anti-BUB1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.

Western blot

Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.
 

Requested From: United States
Date Requested: 12/11/2016

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