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Anti-BUB1 Antibody (clone 4C3-4C9) LS-C196778

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BUB1 Antibodies

Anti-BUB1 Antibody (N-Terminus) IHC-plus™ LS-B2108
Rabbit Polyclonal (IgG) to Human BUB1
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-BUB1 Antibody (aa1-30) LS-C156894
Rabbit Polyclonal to Human BUB1
Human
Western blot, Flow Cytometry
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 4C3-4C9] (IgG1,k) to Human BUB1
Human
Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human BUB1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [4C3-4C9]
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

BUB1 antibody was raised against bUB1 (AAH28201.1, aa1-1085 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human BUB1
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About BUB1

O43683 NM_004336 NP_004327.1

BUB1 Antibody, Bub1A Antibody, BUB1L Antibody, HBUB1 Antibody

Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to BUB1 on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western blot
Western Blot analysis of BUB1 expression in transfected 293T cell line by BUB1 monoclonal antibody (M01), clone 4C3-4C9.Lane 1: BUB1 transfected lysate(122.4 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged BUB1 is approximately 0.03 ng/ml as a capture antibody.
 

Requested From: United States
Date Requested: 12/2/2016

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