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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BTK Antibody (phospho‑Tyr223) LS‑C180161
BTK antibody LS-C180161 is an unconjugated rabbit polyclonal antibody to BTK from human and mouse. Validated for ICC, IHC and WB.
Catalog
Size
Price
LS-C180161-100
100 µl (1.09 mg/ml)
Unavailable

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Product Description

BTK antibody LS-C180161 is an unconjugated rabbit polyclonal antibody to BTK from human and mouse. Validated for ICC, IHC and WB.
About BTK
Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. Q06187 NM_000061 NP_000052.1

BTK Antibody, AGMX1 Antibody, AT Antibody, ATK Antibody, XLA Antibody, PSCTK1 Antibody, B-cell progenitor kinase Antibody, BPK Antibody, Bruton tyrosine kinase Antibody, Tyrosine-protein kinase BTK Antibody, IMD1 Antibody


Specifications

Target
Human BTK
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • ICC (1:200)
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BTK antibody was raised against a synthetic phosphorylated peptide made to a peptide surrounding amino acid 223 of the human BTK protein [UniProt Q06187].
Epitope
pTyr223
Presentation
PBS, 30% glycerol, 0.05% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Immunohistochemistry: BTK [p Tyr223] Antibody - IHC of BTK in kidney cancer xenograft using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry: BTK [p Tyr223] Antibody - IHC of BTK in kidney cancer xenograft using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Immunocytochemistry/Immunofluorescence: BTK [p Tyr223] Antibody - Antibody was tested at 1:50 in Jurkat cells with FITC (green). Nuclei were counterstained with DAPI (blue).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: BTK [p Tyr223] Antibody - Antibody was tested at 1:50 in Jurkat cells with FITC (green). Nuclei were counterstained with DAPI (blue). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot: BTK [p Tyr223] Antibody - WB analysis of BTKpY223 with peptide competition: 1. ab only, 2. 200 molar excess unmodified immunizing peptide and 3. 200 molar excess modified immunizing peptide.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot: BTK [p Tyr223] Antibody - WB analysis of BTKpY223 with peptide competition: 1. ab only, 2. 200 molar excess unmodified immunizing peptide and 3. 200 molar excess modified immunizing peptide. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry: BTK [p Tyr223] Antibody - IHC of BTK in kidney cancer xenograft using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry: BTK [p Tyr223] Antibody - IHC of BTK in kidney cancer xenograft using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Immunocytochemistry/Immunofluorescence: BTK [p Tyr223] Antibody - Antibody was tested at 1:50 in Jurkat cells with FITC (green). Nuclei were counterstained with DAPI (blue).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: BTK [p Tyr223] Antibody - Antibody was tested at 1:50 in Jurkat cells with FITC (green). Nuclei were counterstained with DAPI (blue). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot: BTK [p Tyr223] Antibody - WB analysis of BTKpY223 with peptide competition: 1. ab only, 2. 200 molar excess unmodified immunizing peptide and 3. 200 molar excess modified immunizing peptide.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot: BTK [p Tyr223] Antibody - WB analysis of BTKpY223 with peptide competition: 1. ab only, 2. 200 molar excess unmodified immunizing peptide and 3. 200 molar excess modified immunizing peptide. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry: BTK [p Tyr223] Antibody - IHC of BTK in kidney cancer xenograft using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry: BTK [p Tyr223] Antibody - IHC of BTK in kidney cancer xenograft using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Immunocytochemistry/Immunofluorescence: BTK [p Tyr223] Antibody - Antibody was tested at 1:50 in Jurkat cells with FITC (green). Nuclei were counterstained with DAPI (blue).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: BTK [p Tyr223] Antibody - Antibody was tested at 1:50 in Jurkat cells with FITC (green). Nuclei were counterstained with DAPI (blue). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot: BTK [p Tyr223] Antibody - WB analysis of BTKpY223 with peptide competition: 1. ab only, 2. 200 molar excess unmodified immunizing peptide and 3. 200 molar excess modified immunizing peptide.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot: BTK [p Tyr223] Antibody - WB analysis of BTKpY223 with peptide competition: 1. ab only, 2. 200 molar excess unmodified immunizing peptide and 3. 200 molar excess modified immunizing peptide. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry: BTK [p Tyr223] Antibody - IHC of BTK in kidney cancer xenograft using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry: BTK [p Tyr223] Antibody - IHC of BTK in kidney cancer xenograft using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Immunocytochemistry/Immunofluorescence: BTK [p Tyr223] Antibody - Antibody was tested at 1:50 in Jurkat cells with FITC (green). Nuclei were counterstained with DAPI (blue).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: BTK [p Tyr223] Antibody - Antibody was tested at 1:50 in Jurkat cells with FITC (green). Nuclei were counterstained with DAPI (blue). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot: BTK [p Tyr223] Antibody - WB analysis of BTKpY223 with peptide competition: 1. ab only, 2. 200 molar excess unmodified immunizing peptide and 3. 200 molar excess modified immunizing peptide.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot: BTK [p Tyr223] Antibody - WB analysis of BTKpY223 with peptide competition: 1. ab only, 2. 200 molar excess unmodified immunizing peptide and 3. 200 molar excess modified immunizing peptide. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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