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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BrdU Antibody LS‑C77286
Antibody
BrdU antibody LS-C77286 is an unconjugated mouse monoclonal antibody to BrdU. Validated for IHC.
Catalog
Size
Price
LS-C77286-400
400 µg
Unavailable

Popular BrdU Products

Brdu-incorporated Hut-78 cells stained with purified MoBU-1, followed by anti-mouse IgG FITC
Species: Human
Applications: IHC, Flow Cytometry
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Applications: IHC, Western blot, Immunoprecipitation, ELISA
Formalin-fixed, paraffin-embedded, BrdU-labeled mouse small intestine stained with peroxidase-conjugate and DAB chromogen. Note nuclear staining of crypt epithelial cells.
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Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Antibody
Species:
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Immunofluorescence microscopy images of paraformaldehyde-fixed, paraffin-embedded pancreas sections stained with antibodies against BrdU (red or pink) and counterstained with DAPI (blue) and imaged with a 40 objective. DAPI stained nuclei (blue) indicate non-dividing cells, immunostained red and pink nuclei indicate actively dividing pancreatic -cells. The antibodies were diluted to 2.7 g/ml. and incubated with tissue sections overnight at 4 degrees. Donkey anti-rabbit secondary antibody was diluted 1:2500.
Species:
Applications: Immunofluorescence

Product Description

BrdU antibody LS-C77286 is an unconjugated mouse monoclonal antibody to BrdU. Validated for IHC.
About BrdU
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. 5-Bromodeoxycytidine is deaminated to form BrdU. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle during which DNA is replicated), substituting for thymidine during DNA replication.

Specifications

Target
BrdU
Host
Mouse
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Specificity
Reacts with BrdU that is used to label S-phase cells and will cross-react with iododeoxyuridine (IodU).
Usage
Suitable for use in Immunohistochemistry. It is useful for cell cycle kinetic studies in normal tissue or cells and solid tumors. BrdU can be incorporated into newly synthesized strand of DNA of S-phase cell in vivo and in vitro. Immunohistochemistry: (staining S-phase cells containing BrdU incorporated DNA).
Presentation
PBS, pH 7.4, 0.05% sodium azide.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/19/2018

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