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BrdU

Bromodeoxyuridine (BrdU)

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. 5-Bromodeoxycytidine is deaminated to form BrdU. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle during which DNA is replicated), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical (see immunohistochemistry), thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat. BrdU can be passed to daughter cells upon replication. BrdU has been demonstrated to be detectable over two years post-infusion.

BrdU Target Details

Target Name: Bromodeoxyuridine (BrdU)

Publications (11)

1
Intrastriatal transforming growth factor alpha delivery to a model of Parkinson's disease induces proliferation and migration of endogenous adult neural progenitor cells without differentiation into dopaminergic neurons. Cooper O, Isacson O. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2004 24:8924-31. [PubMed:15483111] [PMC:PMC2613225]
2
Kaposi's sarcoma-associated herpesvirus ori-Lyt-dependent DNA replication: dual role of replication and transcription activator. Wang Y, Tang Q, Maul GG, Yuan Y. Journal of virology. 2006 80:12171-86. (ICC) [PubMed:17020951] [PMC:PMC1676287]
3
CDH1 is a specific marker for undifferentiated spermatogonia in mouse testes. Tokuda M, Kadokawa Y, Kurahashi H, Marunouchi T. Biology of reproduction. 2007 76:130-41. (ICC) [PubMed:17035642]
4
Expansion of peripheral naturally occurring T regulatory cells by Fms-like tyrosine kinase 3 ligand treatment. Swee LK, Bosco N, Malissen B, Ceredig R, Rolink A. Blood. 2009 113:6277-87. [PubMed:19211508] Related Antibodies: LS-C40882.
5
IL-11 regulates autoimmune demyelination. Gurfein BT, Zhang Y, Lpez CB, Argaw AT, Zameer A, Moran TM, John GR. Journal of immunology (Baltimore, Md. : 1950). 2009 183:4229-40. [PubMed:19734214] [PMC:PMC3027139]
6
Notch1 signaling plays a role in regulating precursor differentiation during CNS remyelination. Zhang Y, Argaw AT, Gurfein BT, Zameer A, Snyder BJ, Ge C, Lu QR, Rowitch DH, Raine CS, Brosnan CF, John GR. Proceedings of the National Academy of Sciences of the United States of America. 2009 106:19162-7. [PubMed:19855010] [PMC:PMC2776461]
7
Environmental complexity, seasonality and brain cell proliferation in a weakly electric fish, Brachyhypopomus gauderio. Dunlap KD, Silva AC, Chung M. The Journal of experimental biology. 2011 214:794-805. (IHC) [PubMed:21307066] [PMC:PMC3036548]
8
Specific inhibition of NF-Y subunits triggers different cell proliferation defects. Benatti P, Dolfini D, Vigan A, Ravo M, Weisz A, Imbriano C. Nucleic acids research. 2011 39:5356-68. [PubMed:21415014] [PMC:PMC3141247] Related Antibodies: LS-C40882.
9
Proapoptotic and antiapoptotic actions of Stat1 versus Stat3 underlie neuroprotective and immunoregulatory functions of IL-11. Zhang J, Zhang Y, Dutta DJ, Argaw AT, Bonnamain V, Seto J, Braun DA, Zameer A, Hayot F, Lpez CB, Raine CS, John GR. Journal of immunology (Baltimore, Md. : 1950). 2011 187:1129-41. [PubMed:21709156] [PMC:PMC3164308]
10
Detection of BrdU-label retaining cells in the lacrimal gland: implications for tissue repair. You S, Tariq A, Kublin CL, Zoukhri D. Cell and tissue research. 2011 346:317-26. [PubMed:22101331] [PMC:PMC3245820]
11
Maternal vitamin C deficiency during pregnancy persistently impairs hippocampal neurogenesis in offspring of guinea pigs. Tveden-Nyborg P, Vogt L, Schjoldager JG, Jeannet N, Hasselholt S, Paidi MD, Christen S, Lykkesfeldt J. PloS one. 2012 7:e48488. (ICC, IHC; Guinea pig) [PubMed:23119033] [PMC:PMC3485340]
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☰ Filters
Antibodies (147)
ELISA Kits (1)
Assay Kits (1)
Competitive EIA (1)
Detection/Quantition (1)
Primary (147)
BrdU (149)
Human (32)
Mouse (1)
Rat (2)
All species (4)
Synthetic (4)
IHC (122)
IHC-Fr (10)
IHC-P (68)
WB (9)
Flo (51)
ELISA (13)
ICC (26)
IF (53)
IP (7)
rabbit (7)
mouse (108)
rat (25)
sheep (7)
IgG (15)
IgG,k (2)
IgG1 (76)
IgG1,k (18)
IgG1,l (1)
IgG2a (5)
IgG2b (25)
monoclonal mc (135)
polyclonal pc (10)
recombinant monoclonal rmc (2)
1C7 (1)
1D7 (1)
24H9 (1)
29G6.E8 (1)
2A6 (1)
2B1 (2)
2B12 (1)
2B5 (1)
2G6 (1)
2H8 (1)
3A4 (1)
3A8 (1)
3B2 (1)
3B6 (1)
3F11 (1)
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4A5 (1)
4A6 (1)
4B6C12 (1)
4C2 (1)
4C9 (1)
4F2 (1)
4G7 (1)
4H10 (1)
5C1 (1)
5E6 (1)
7F12 (1)
7H4 (1)
7H5 (1)
85-2C8 (3)
8B8 (1)
9E8 (1)
B33.1 (1)
BRD.3 (3)
BRD2888R (4)
BRD469 (2)
BRD469 + BRD494 + BRD.3 (3)
BRD494 (3)
BrdU 206 (2)
Bu20a (4)
BU20a (3)
BU33 (1)
BU5.1 (1)
BU6-4 (1)
IIB5 (2)
MoBU (1)
MoBu-1 (3)
MoBU-1 (3)
OTI1C7 (1)
OTI1D7 (1)
OTI1E2 (1)
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OTI5C1 (1)
OTI5E6 (1)
OTI7H4 (2)
OTI7H5 (1)
OTI8B8 (1)
SPM166 (2)
SPM537 (2)
96-Well Strip Plate (1)
AP Conjugated (3)
APC Conjugated (2)
Azide-free (11)
Biotin Conjugated (5)
BSA-free (10)
Carrier-free (31)
FITC Conjugated (8)
HRP Conjugated (4)
PE Conjugated (5)
Ready-to-Use (6)
Unconjugated (120)
No (148)
Yes (1)
IHC-Fr (1)
IHC-P (1)
Plasma (1)
Serum (1)
BrdU Antibody - Immunofluorescence microscopy images of paraformaldehyde-fixed, paraffin-embedded pancreas sections stained with antibodies against BrdU (red or pink) and counterstained with DAPI (blue) and imaged with a 40 objective. DAPI stained nuclei (blue) indicate non-dividing cells, immunostained red and pink nuclei indicate actively dividing pancreatic -cells. The antibodies were diluted to 2.7 g/ml. and incubated with tissue sections overnight at 4 degrees. Donkey anti-rabbit secondary antibody was diluted 1:2500.
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BrdU Rabbit Polyclonal Antibody
ELISA, Flo, IF, IHC, IP, WB
Unconjugated
100 µg/$567
BrdU Antibody - Brdu-incorporated Hut-78 cells stained with purified MoBU-1, followed by anti-mouse IgG FITC
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BrdU Mouse Monoclonal (MoBU-1) Antibody
Human
Flo, IHC
Unconjugated
100 µg/$287
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BrdU Sheep Polyclonal Antibody
ELISA, IHC, IP, WB
Unconjugated
250 µg/$916
BrdU Antibody - IHC of paraffin-embedded colon tissue from BrdU injected mouse using anti-BrdU rat monoclonal antibody.
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BrdU Rat Monoclonal (2H8) Antibody
Human
IHC, IHC-P
Unconjugated
100 µl/$434
BrdU Antibody - Immunofluorescence Microscopy of rabbit anti-BrdU antibody. Tissue: 293T cells transfected expressing GFP-MCM9 L. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: BrdU antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Anti-rabbit ATTO550 secondary antibody at 1:10,000 for 45 min at RT. Localization: BrdU is nuclear. Staining: BrdU in merged image shows with green and purple fluorescent signal with DAPI nuclear counterstain.
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BrdU Rabbit Polyclonal Antibody
ELISA, Flo, IF, IHC, IP, WB
Unconjugated
25 µl/$304
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BrdU Mouse Monoclonal (B33.1) Antibody
Human
IHC
Unconjugated
0.5 ml/$328
BrdU Antibody - IHC of paraffin-embedded colon tissue from BrdU injected mouse using anti-BrdU mouse monoclonal antibody.
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BrdU Mouse Monoclonal (2G6) Antibody
ICC, IF, IHC, IHC-P
Unconjugated
100 µl/$434
BrdU Antibody - IHC of paraffin-embedded colon tissue from BrdU injected mouse using anti-BrdU mouse monoclonal antibody.
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BrdU Mouse Monoclonal (7F12) Antibody
ICC, IF, IHC, IHC-P
Unconjugated
100 µl/$434
BrdU Antibody - IHC of paraffin-embedded colon tissue from BrdU injected mouse using anti-BrdU mouse monoclonal antibody.
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BrdU Mouse Monoclonal (4H10) Antibody
ICC, IF, IHC, IHC-P
Unconjugated
100 µl/$434
BrdU Antibody - IHC of paraffin-embedded colon tissue from BrdU injected mouse using anti-BrdU mouse monoclonal antibody.
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BrdU Mouse Monoclonal (7H4) Antibody
ICC, IF, IHC, IHC-P
Unconjugated
100 µl/$434
BrdU Antibody - IHC of paraffin-embedded colon tissue from BrdU injected mouse using anti-BrdU mouse monoclonal antibody.
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BrdU Mouse Monoclonal (2B5) Antibody
ICC, IF, IHC, IHC-P
Unconjugated
100 µl/$434
BrdU Antibody - IHC of paraffin-embedded colon tissue from BrdU injected mouse using anti-BrdU mouse monoclonal antibody.
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BrdU Mouse Monoclonal (1C7) Antibody
ICC, IF, IHC, IHC-P
Unconjugated
100 µl/$434
BrdU Antibody - IHC of paraffin-embedded colon tissue from BrdU injected mouse using anti-BrdU mouse monoclonal antibody.
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BrdU Mouse Monoclonal (4A3) Antibody
ICC, IF, IHC, IHC-P
Unconjugated
100 µl/$434
BrdU Antibody - Immunofluorescence Microscopy of Mouse Anti-BrdU antibody. Tissue: OCT-embedded E10.5 mouse embryo. Localization: 40X, section through the developing limb bud. Fixation: 4% PFA. Antigen retrieval: not required. Primary antibody: BrdU antibody at 1:500 in 0.4% PBS+Triton with 1% normal sheep serum overnight at 4 degrees C. Secondary antibody: Alexa Fluor 488 Anti-Mouse secondary antibody at 1:200 for 45 min at RT. Staining: Double labeled (green/blue) cells represent cells that were actively dividing.
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BrdU Mouse Monoclonal (29G6.E8) Antibody
ELISA, Flo, IF, IHC, WB
Unconjugated
100 µg/$574
BrdU Antibody - IHC of paraffin-embedded colon tissue from BrdU injected mouse using anti-BrdU mouse monoclonal antibody.
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BrdU Mouse Monoclonal (3A4) Antibody
ICC, IF, IHC, IHC-P
Unconjugated
100 µl/$434
BrdU Antibody - IHC of paraffin-embedded colon tissue from BrdU injected mouse using anti-BrdU mouse monoclonal antibody.
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BrdU Mouse Monoclonal (5C1) Antibody
ICC, IF, IHC, IHC-P
Unconjugated
100 µl/$434
BrdU Antibody - IHC of paraffin-embedded colon tissue from BrdU injected mouse using anti-BrdU mouse monoclonal antibody.
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BrdU Mouse Monoclonal (2B12) Antibody
ICC, IF, IHC, IHC-P
Unconjugated
100 µl/$434
BrdU Antibody - BrdU-labeled human lymphoma cells were stained with rabbit anti-BrdU antibody at a 1/50 dilution and a 1/100 dilution. Sheep anti Rabbit IgG DyLight® 488 conjugated antibody was used as the secondary detection reagent at a 1/50 dilution. READIDROP™ Propidium Iodide was used to stain total DNA.
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BrdU Rabbit anti-Synthetic Polyclonal Antibody
Synthetic
Flo, ICC, IF, IHC-P
Unconjugated
50 µg/$448
BrdU Antibody - Detection of BrdU incorporation into pulsed-labeled ACNC12 cells using Mouse anti-BrdU antibody (clone Bu20a, MCA2483).
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BrdU Mouse anti-Synthetic Monoclonal (Bu20a) Antibody
Synthetic
Flo, IF, IHC, IHC-Fr, IHC-P
Unconjugated
20 µg/$285; 0.1 mg/$395; 0.2 mg/$486
BrdU Antibody - BrdU antibody BRD494 immunohistochemistry intestine 10X
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BrdU Mouse Monoclonal (BRD494) Antibody
Flo, IF, IHC, IHC-P
Unconjugated
20 µg/$371; 100 µg/$574
BrdU Antibody - BrdU antibody BRD494 immunohistochemistry intestine 10X This image was taken for the unmodified form of this product. Other forms have not been tested.
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BrdU Mouse Monoclonal (Azide-free, BSA-free) (BRD494) Antibody
Flo, IF, IHC, IHC-P
Unconjugated, Azide-free, BSA-free
100 µg/$574
BrdU Antibody - Formalin-fixed, paraffin-embedded mouse small intestine stained with Bromodeoxyuridine antibody (MoBu-1). This image was taken for the unmodified form of this product. Other forms have not been tested.
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BrdU Mouse Monoclonal (MoBu-1) Antibody
Flo, IF, IHC, IHC-P
Unconjugated
20 µg/$371; 100 µg/$574
BrdU Antibody - Formalin-fixed, paraffin-embedded mouse small intestine stained with BrdU antibody (BRD.3). This image was taken for the unmodified form of this product. Other forms have not been tested.
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BrdU Mouse Monoclonal (BRD.3) Antibody
Flo, IF, IHC, IHC-P
Unconjugated
20 µg/$371; 100 µg/$574
BrdU Antibody - Formalin-fixed, paraffin-embedded mouse small intestine stained with BrdU antibody (BU20a). This image was taken for the unmodified form of this product. Other forms have not been tested.
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BrdU Mouse Monoclonal (BU20a) Antibody
Flo, IF, IHC, IHC-P
Unconjugated
20 µg/$371; 100 µg/$574
BrdU Antibody - Formalin-fixed, paraffin-embedded mouse small intestine stained with BrdU antibody (BU20a). This image was taken for the unmodified form of this product. Other forms have not been tested.
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BrdU Mouse Monoclonal (Azide-free, BSA-free) (BU20a) Antibody
Flo, IF, IHC, IHC-P
Unconjugated, Azide-free, BSA-free
100 µg/$574
Viewing 1-25 of 149 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).