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Anti-BLIMP1 / PRDM1 Antibody (clone 6D3) LS-C107090

Ordering

Wt. Vol. Conc. Price
25 µg - - $235
100 µg - - $345
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular BLIMP1 / PRDM1 Antibodies

Anti-BLIMP1 / PRDM1 Antibody (C-Terminus) IHC-plus™ LS-B2722
Rabbit Polyclonal to Human BLIMP1 / PRDM1
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-BLIMP1 / PRDM1 Antibody (aa611-660) IHC-plus™ LS-B4694
Rabbit Polyclonal to Human BLIMP1 / PRDM1
Human, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal [clone 6D3] (IgG2a) to Mouse BLIMP1 / PRDM1
Mouse, Human
IHC - Paraffin, Western blot
Unconjugated

Details

Mouse BLIMP1 / PRDM1
Rat
Mouse, Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [6D3]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin
  • Western blot

Specificity and Use

BLIMP1 / PRDM1 antibody was raised against mouse PRDM1
This 6D3 antibody has been tested by western blot of reduced A20 cell lysates. For western blotting, this antibody can be used at 1-10 ug/mL. Moreover, this antibody was tested by immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue. Antigen retrieval was performed using 10 mM citrate buffer at pH 6.0. For immunohistochemistry, this antibody can be used at 10 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging

Aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Store at 4°C, avoid repeated freeze thaw cycles.
For research use only.

About BLIMP1 / PRDM1

O75626 NM_001198 NP_001189.2

PRDM1 Antibody, BLIMP-1 Antibody, BLIMP1 Antibody, PRDI-binding factor-1 Antibody, PRDI-BF1 Antibody, PR domain-containing protein 1 Antibody, PRDI-binding factor 1 Antibody

Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569).

Immunohistochemistry

Immunohistochemistry
Immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue with citrate buffer antigen retrieval, using 10 ug/ml of anti-mouse/human Blimp-1 (6D3) (left) or anti-rat IgG2a antibody (right) followed by biotinylated anti-rat IgG, and DAB visualization.

Western blot

Western blot
Western Blot of reduced cell lysates prepared from A20 cells using 5 ug/mL anti-mouse/human Blimp-1 (6D3) antibody. Bands were visualized using HRP-conjugated rat anti-mouse IgG antibody.

Requested From: United States
Date Requested: 12/7/2016

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