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Anti-BLIMP1 / PRDM1 Antibody (clone 6D3) LS-C107090

Catalog Size Price
LS-C107090-25 25 µg Unavailable
LS-C107090-100 100 µg Unavailable

Related Products

46 BLIMP1 / PRDM1 Antibodies

Most Popular BLIMP1 / PRDM1 Antibodies

Anti-BLIMP1 / PRDM1 Antibody (aa199-409, clone 3H2-E8) LS-C2538
Mouse Monoclonal [clone 3H2-E8] (IgG1) to Mouse BLIMP1 / PRDM1
Mouse, Human, Pig
ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10mum). Positive surface epithelium and centrocytes are labeled.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-BLIMP1 / PRDM1 Antibody (aa37-86) LS-C31422
Rabbit Polyclonal (IgG) to Human BLIMP1 / PRDM1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
Western blot
Unconjugated
A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-BLIMP1 / PRDM1 Antibody (C-Terminus) IHC-plus™ LS-B2722
Rabbit Polyclonal (IgG) to Human BLIMP1 / PRDM1
Human, Mouse, Rat
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-BLIMP-1 antibody IHC of human colon, lymphoid follicle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2722 concentration 3 ug/ml.
Anti-BLIMP1 / PRDM1 Antibody (aa611-660) IHC-plus™ LS-B4694
Rabbit Polyclonal to Human BLIMP1 / PRDM1
Human, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-BLIMP-1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4694 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-BLIMP1 / PRDM1 Antibody (C-Terminus) IHC-plus™ LS-B10615
Rabbit Polyclonal (IgG) to Human BLIMP1 / PRDM1
Human, Mouse, Rat, Dog
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-BLIMP1 / PRDM1 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

100% Guaranteed
Rat Monoclonal [clone 6D3] (IgG2a) to Mouse BLIMP1 / PRDM1
Mouse, Human
IHC, IHC - Paraffin, Western blot
Unconjugated

Details

Mouse BLIMP1 / PRDM1
Rat
Mouse, Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [6D3]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin
  • Western blot

Specificity and Use

BLIMP1 / PRDM1 antibody was raised against mouse PRDM1
This 6D3 antibody has been tested by western blot of reduced A20 cell lysates. For western blotting, this antibody can be used at 1-10 ug/mL. Moreover, this antibody was tested by immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue. Antigen retrieval was performed using 10 mM citrate buffer at pH 6.0. For immunohistochemistry, this antibody can be used at 10 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging

Aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Store at 4°C, avoid repeated freeze thaw cycles.
For research use only.

About BLIMP1 / PRDM1

O75626 NM_001198 NP_001189.2

PRDM1 Antibody, BLIMP-1 Antibody, BLIMP1 Antibody, PRDI-binding factor-1 Antibody, PRDI-BF1 Antibody, PR domain-containing protein 1 Antibody, PRDI-binding factor 1 Antibody

Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569).

Immunohistochemistry

Immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue with citrate buffer antigen retrieval, using 10 ug/ml of anti-mouse/human Blimp-1 (6D3) (left) or anti-rat IgG2a antibody (right) followed by biotinylated anti-rat IgG, and DAB visualization.
Immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue with citrate buffer antigen retrieval, using 10 ug/ml of anti-mouse/human Blimp-1 (6D3) (left) or anti-rat IgG2a antibody (right) followed by biotinylated anti-rat IgG, and DAB visualization.

Western blot

Western Blot of reduced cell lysates prepared from A20 cells using 5 ug/mL anti-mouse/human Blimp-1 (6D3) antibody. Bands were visualized using HRP-conjugated rat anti-mouse IgG antibody.
Western Blot of reduced cell lysates prepared from A20 cells using 5 ug/mL anti-mouse/human Blimp-1 (6D3) antibody. Bands were visualized using HRP-conjugated rat anti-mouse IgG antibody.

Requested From: 
Date Requested: 4/22/2018

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