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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BLIMP1 / PRDM1 Antibody (clone 6D3) LS‑C107090
PRDM1 antibody LS-C107090 is an unconjugated rat monoclonal antibody to PRDM1 (BLIMP1) from human and mouse. Validated for IHC and WB.
Catalog
Size
Price
LS-C107090-25
25 µg
Unavailable
LS-C107090-100
100 µg
Unavailable

Popular BLIMP1 / PRDM1 Products

Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10mum). Positive surface epithelium and centrocytes are labeled.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human, Pig
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
Applications: Western blot
Anti-BLIMP-1 antibody IHC of human colon, lymphoid follicle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2722 concentration 3 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Anti-BLIMP-1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4694 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Anti-BLIMP1 / PRDM1 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Mouse, Rat, Dog
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA

Product Description

PRDM1 antibody LS-C107090 is an unconjugated rat monoclonal antibody to PRDM1 (BLIMP1) from human and mouse. Validated for IHC and WB.
About BLIMP1 / PRDM1
Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569). O75626 NM_001198 NP_001189.2

PRDM1 Antibody, BLIMP-1 Antibody, BLIMP1 Antibody, PRDI-binding factor-1 Antibody, PRDI-BF1 Antibody, PR domain-containing protein 1 Antibody, PRDI-binding factor 1 Antibody


Specifications

Target
Mouse BLIMP1 / PRDM1
Host
Rat
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [6D3]
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BLIMP1 / PRDM1 antibody was raised against mouse PRDM1
Usage
This 6D3 antibody has been tested by western blot of reduced A20 cell lysates. For western blotting, this antibody can be used at 1-10 ug/mL. Moreover, this antibody was tested by immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue. Antigen retrieval was performed using 10 mM citrate buffer at pH 6.0. For immunohistochemistry, this antibody can be used at 10 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Presentation
Aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage
Store at 4°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue with citrate buffer antigen retrieval, using 10 ug/ml of anti-mouse/human Blimp-1 (6D3) (left) or anti-rat IgG2a antibody (right) followed by biotinylated anti-rat IgG, and DAB visualization.
Immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue with citrate buffer antigen retrieval, using 10 ug/ml of anti-mouse/human Blimp-1 (6D3) (left) or anti-rat IgG2a antibody (right) followed by biotinylated anti-rat IgG, and DAB visualization.

Western blot

Western Blot of reduced cell lysates prepared from A20 cells using 5 ug/mL anti-mouse/human Blimp-1 (6D3) antibody. Bands were visualized using HRP-conjugated rat anti-mouse IgG antibody.
Western Blot of reduced cell lysates prepared from A20 cells using 5 ug/mL anti-mouse/human Blimp-1 (6D3) antibody. Bands were visualized using HRP-conjugated rat anti-mouse IgG antibody.

Immunohistochemistry

Immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue with citrate buffer antigen retrieval, using 10 ug/ml of anti-mouse/human Blimp-1 (6D3) (left) or anti-rat IgG2a antibody (right) followed by biotinylated anti-rat IgG, and DAB visualization.
Immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue with citrate buffer antigen retrieval, using 10 ug/ml of anti-mouse/human Blimp-1 (6D3) (left) or anti-rat IgG2a antibody (right) followed by biotinylated anti-rat IgG, and DAB visualization.

Western blot

Western Blot of reduced cell lysates prepared from A20 cells using 5 ug/mL anti-mouse/human Blimp-1 (6D3) antibody. Bands were visualized using HRP-conjugated rat anti-mouse IgG antibody.
Western Blot of reduced cell lysates prepared from A20 cells using 5 ug/mL anti-mouse/human Blimp-1 (6D3) antibody. Bands were visualized using HRP-conjugated rat anti-mouse IgG antibody.

Immunohistochemistry

Immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue with citrate buffer antigen retrieval, using 10 ug/ml of anti-mouse/human Blimp-1 (6D3) (left) or anti-rat IgG2a antibody (right) followed by biotinylated anti-rat IgG, and DAB visualization.
Immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue with citrate buffer antigen retrieval, using 10 ug/ml of anti-mouse/human Blimp-1 (6D3) (left) or anti-rat IgG2a antibody (right) followed by biotinylated anti-rat IgG, and DAB visualization.

Western blot

Western Blot of reduced cell lysates prepared from A20 cells using 5 ug/mL anti-mouse/human Blimp-1 (6D3) antibody. Bands were visualized using HRP-conjugated rat anti-mouse IgG antibody.
Western Blot of reduced cell lysates prepared from A20 cells using 5 ug/mL anti-mouse/human Blimp-1 (6D3) antibody. Bands were visualized using HRP-conjugated rat anti-mouse IgG antibody.

Immunohistochemistry

Immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue with citrate buffer antigen retrieval, using 10 ug/ml of anti-mouse/human Blimp-1 (6D3) (left) or anti-rat IgG2a antibody (right) followed by biotinylated anti-rat IgG, and DAB visualization.
Immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue with citrate buffer antigen retrieval, using 10 ug/ml of anti-mouse/human Blimp-1 (6D3) (left) or anti-rat IgG2a antibody (right) followed by biotinylated anti-rat IgG, and DAB visualization.

Western blot

Western Blot of reduced cell lysates prepared from A20 cells using 5 ug/mL anti-mouse/human Blimp-1 (6D3) antibody. Bands were visualized using HRP-conjugated rat anti-mouse IgG antibody.
Western Blot of reduced cell lysates prepared from A20 cells using 5 ug/mL anti-mouse/human Blimp-1 (6D3) antibody. Bands were visualized using HRP-conjugated rat anti-mouse IgG antibody.


Requested From: 
Date Requested: 6/24/2018

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