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Catalog Number Size Price
LS-B8314-50 50 µg (0.5 mg/ml) $485 
BLIMP1 / PRDM1 Antibody - Anti-BLIMP1 / PRDM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
BLIMP1 / PRDM1 Antibody - PRDM1 / MEL1 Antibody Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
BLIMP1 / PRDM1 Antibody - PRDM1 / MEL1 Antibody (0.1µg/ml) staining of A431 (A), (2ug/ml) K562 (B) and (1ug/ml) Nuclear K562 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescencence.
BLIMP1 / PRDM1 Antibody - BLIMP1 / PRDM1 antibody (0.5µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
BLIMP1 / PRDM1 Antibody - PRDM1 antibody (0.3 ug/ml) staining of A431 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
BLIMP1 / PRDM1 Antibody - PRDM1 / MEL1 Antibody Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
BLIMP1 / PRDM1 Antibody - Anti-BLIMP1 / PRDM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
BLIMP1 / PRDM1 Antibody - PRDM1 / MEL1 Antibody Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
BLIMP1 / PRDM1 Antibody - PRDM1 / MEL1 Antibody (0.1µg/ml) staining of A431 (A), (2ug/ml) K562 (B) and (1ug/ml) Nuclear K562 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescencence.
BLIMP1 / PRDM1 Antibody - BLIMP1 / PRDM1 antibody (0.5µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
BLIMP1 / PRDM1 Antibody - PRDM1 antibody (0.3 ug/ml) staining of A431 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
BLIMP1 / PRDM1 Antibody - PRDM1 / MEL1 Antibody Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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IHC‑plus™ Polyclonal Goat anti‑Human BLIMP1 / PRDM1 Antibody (aa698‑711, IHC, IF, WB) LS‑B8314

IHC‑plus™ Polyclonal Goat anti‑Human BLIMP1 / PRDM1 Antibody (aa698‑711, IHC, IF, WB) LS‑B8314

Note: This antibody replaces LS-C112900
Antibody:
BLIMP1 / PRDM1 Goat anti-Human Polyclonal (aa698-711) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B8314-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BLIMP1 / PRDM1 Goat anti-Human Polyclonal (aa698-711) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
PRDM1 antibody LS-B8314 is an unconjugated goat polyclonal antibody to PRDM1 (BLIMP1) (aa698-711) from human. It is reactive with human, monkey and rabbit. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BLIMP1 / PRDM1
Synonyms
PRDM1 | BLIMP-1 | BLIMP1 | PRDI-binding factor-1 | PRDI-BF1 | PR domain-containing protein 1 | PRDI-binding factor 1
Host
Goat
Reactivity
Human, Monkey, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-DISDNADRLEDVED, from the internal region of the protein sequence according to NP_001189.2NP_878911.1.
Epitope
aa698-711
Specificity
Human PRDM1 / BLIMP-1. This antibody is expected to recognize both reported isoforms (NP_001189.2; NP_878911.1).
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (0.1 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 90kDa band observed in lysates of cell lines A431 and A549 (calculated MW of 91.8kDa according to NP_001189.2). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BLIMP1 / PRDM1
O75626 NM_001198 NP_001189.2

Validation

BLIMP1 / PRDM1 Antibody - Anti-BLIMP1 / PRDM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-BLIMP1 / PRDM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-BLIMP1 / PRDM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

LSBio Ratings

IHC-plus™ BLIMP1 / PRDM1 Antibody (aa698-711) for IHC, IF/Immunofluorescence, WB/Western LS-B8314 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024