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IHCPlus™ BLIMP1 / PRDM1 Antibody aa698‑711 Polyclonal IF,IHC,Peptide‑ELISA,WB LS‑B8314

Catalog Number Size Price
LS-B8314-50 50 µg (0.5 mg/ml) $425 
Anti-BLIMP1 / PRDM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
PRDM1 / MEL1 Antibody Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
PRDM1 antibody (0.3 ug/ml) staining of A431 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PRDM1 / MEL1 Antibody (0.1µg/ml) staining of A431 (A), (2ug/ml) K562 (B) and (1ug/ml) Nuclear K562 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescencence.
BLIMP1 / PRDM1 antibody (0.5µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
PRDM1 / MEL1 Antibody Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Anti-BLIMP1 / PRDM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
PRDM1 / MEL1 Antibody Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
PRDM1 antibody (0.3 ug/ml) staining of A431 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PRDM1 / MEL1 Antibody (0.1µg/ml) staining of A431 (A), (2ug/ml) K562 (B) and (1ug/ml) Nuclear K562 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescencence.
BLIMP1 / PRDM1 antibody (0.5µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
PRDM1 / MEL1 Antibody Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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IHCPlus™ BLIMP1 / PRDM1 Antibody aa698‑711 Polyclonal IF,IHC,Peptide‑ELISA,WB LS‑B8314

Note: This antibody replaces LS-C112900
Antibody:
Goat Polyclonal to Human BLIMP1 / PRDM1
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8314-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human BLIMP1 / PRDM1
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
PRDM1 antibody LS-B8314 is an unconjugated goat polyclonal antibody to PRDM1 (BLIMP1 ) from human, monkey and rabbit. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BLIMP1 / PRDM1
Host
Goat
Reactivity
Human, Monkey, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (0.1 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-DISDNADRLEDVED, from the internal region of the protein sequence according to NP_001189.2NP_878911.1.
Blocking Peptide
LS-E27216 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8314. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa698-711
Specificity
Human PRDM1 / BLIMP-1. This antibody is expected to recognize both reported isoforms (NP_001189.2; NP_878911.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 90kDa band observed in lysates of cell lines A431 and A549 (calculated MW of 91.8kDa according to NP_001189.2). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BLIMP1 / PRDM1
O75626 NM_001198 NP_001189.2

Validation

Anti-BLIMP1 / PRDM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
IHC Validated
Anti-BLIMP1 / PRDM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-BLIMP1 / PRDM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

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Requested From: United States
Date Requested: 8/23/2019
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