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Anti-Biotin Antibody (HRP) LS-C77034

Ordering

Wt. Vol. Conc. Price
1000 µg - 10 mg/ml $245
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Biotin Antibodies

Anti-Biotin Antibody LS-C194408
Mouse Monoclonal (IgG1)
ELISA
Unconjugated
Anti-Biotin Antibody (HRP) LS-C77034
Rabbit Polyclonal (IgG)
IHC, Western blot, ELISA
HRP Conjugated
Anti-Biotin Antibody (Cy5) LS-C77018
Goat Polyclonal
Immunofluorescence, Flow Cytometry, ELISA
Cy5 Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG)
IHC, Western blot, ELISA
HRP Conjugated

Details

Biotin
Rabbit
IgG Polyclonal
Horseradish Peroxidase
Ion exchange chromatography
Unmodified

Applications

  • IHC
  • Western blot
  • ELISA (1:6000 - 1:25000)

Specificity and Use

Biotin antibody was raised against biotin conjugated to KLH.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti peroxidase, anti rabbit serum, biotinylated IgG and biotinylated BSA.
Suitable for use in Western Blot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. ELISA: 1:6000-1:25000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

PBS, pH 7.2, BSA (IgG, protease free), 40% glycerol, 0.01% Gentamicin sulfate.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About Biotin

Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size means the biological activity of the protein will most likely be unaffected. This process is called biotinylation. Because both streptavidin and avidin bind biotin with high affinity (Kd of 10-14 mol/l to 10-15 mol/l) and specificity, biotinylated proteins of interest can be isolated from a sample by exploiting this highly stable interaction.

Requested From: United States
Date Requested: 12/7/2016

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