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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BABAM1 / HSPC142 Antibody (aa36‑85) IHC‑plus™ LS‑B8224 Best Seller
Note: This antibody replaces LS-C81973
HSPC142 antibody LS-B8224 is an unconjugated rabbit polyclonal antibody to HSPC142 (BABAM1) from human, mouse, dog and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B8224-50
50 µg (1 mg/ml)
Unavailable

Popular BABAM1 / HSPC142 Products

Formalin-fixed and paraffin-embedded human brain reacted with HSPC142 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat, Bovine, Dog, Horse, Pig
Applications: IHC, IHC - Paraffin, Immunofluorescence, Immunoprecipitation
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat, Bovine, Dog, Horse, Pig
Applications: IHC, IHC - Paraffin, Immunofluorescence

Product Description

HSPC142 antibody LS-B8224 is an unconjugated rabbit polyclonal antibody to HSPC142 (BABAM1) from human, mouse, dog and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About BABAM1 / HSPC142
Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Q9NWV8 NM_014173 NP_054892.2

BABAM1 Antibody, C19orf62 Antibody, NBA1 Antibody, HSPC142 Antibody, MERIT40 Antibody


Specifications

Target
Human BABAM1 / HSPC142
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Dog, Guinea pig, Horse (tested or 100% immunogen sequence identity)
Predicted
Rat, Bovine, Xenopus (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 - 7.5 µg/ml)
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa36-85 of human BABAM1 (Q9NWV8, NP_054892). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Panda, Dog, Horse, Guinea pig (100%); Galago, Rat, Elephant, Bovine, Opossum, Xenopus (92%).
Blocking Peptide
LS-E19837 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8224. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa36-85
Specificity
Human BABAM1 / HSPC142
Usage
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-BABAM1 / HSPC142 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8224 dilution 5-7.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-BABAM1 / HSPC142 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8224 dilution 5-7.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-BABAM1 / HSPC142 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8224 dilution 5-7.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-BABAM1 / HSPC142 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8224 dilution 5-7.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-BABAM1 / HSPC142 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8224 dilution 5-7.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-BABAM1 / HSPC142 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8224 dilution 5-7.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-BABAM1 / HSPC142 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8224 dilution 5-7.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-BABAM1 / HSPC142 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8224 dilution 5-7.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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