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Anti-B2M / Beta 2 Microglobulin Antibody (clone B2M-01) IHC-plus™ LS-B8113

Note: This antibody replaces LS-C153035


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular B2M / Beta 2 Microglobulin Antibodies

Anti-B2M / Beta 2 Microglobulin Antibody (clone B2M-01, HRP) LS-C46548
Mouse Monoclonal [clone B2M-01] (IgG2a) to Human B2M / Beta 2 Microglobulin
Western blot
HRP Conjugated
Anti-B2M / Beta 2 Microglobulin Antibody (clone 2M2) IHC-plus™ LS-B2200
Mouse Monoclonal [clone 2M2] (IgG1) to Human B2M / Beta 2 Microglobulin
IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Immunohistochemistry Image
Anti-B2M / Beta 2 Microglobulin Antibody (clone 2213) IHC-plus™ LS-B2249
Mouse Monoclonal [clone 2213] (IgG1) to Human B2M / Beta 2 Microglobulin
IHC - Paraffin, ELISA
Immunohistochemistry Image

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Mouse Monoclonal [clone B2M-01] (IgG2a) to Human B2M / Beta 2 Microglobulin
IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Radioimmunoassay


Human B2M / Beta 2 Microglobulin
Human (tested or 100% immunogen sequence identity)
Mouse, Bovine, Dog, Rabbit, Chicken
IgG2a Monoclonal [B2M-01]


  • IHC - Paraffin (1:50)
  • Western blot (2 - 4 µg/ml)
  • Immunoprecipitation
  • Flow Cytometry
  • Radioimmunoassay

Specificity and Use

B2M / Beta 2 Microglobulin antibody was raised against purified human beta2-microglobulin
Reacts with beta2-microglobulin (b2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. b2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes,thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
For use in immunoprecipitation, this antibody coprecipitates b2-microglobulin (12 kDa) and HLA antigens(45 kDa) from the lysates of human lymphoid cells. Recommend U937 human histiocytic lymphoma cell line orRAJI human Burkitt lymphoma cell line as postitive control and EL4 mouse lymphoblastic lymphoma cell line as negative control for Western blot. Reducing and non-reducing conditions. Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing/reducing Laemmli SDS-PAGE sample buffer. Recommend peripheral blood lymphocytes as positve control and DAUDI human Burkitt lymphoma cell line, or erythrocytes as negative control for flow cytometry. Recommend kidney glomeruli as postive control for IHC. For RIA, use at an assay dependent concentration. The dissociation constant of the antibody soluble b2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA.


PBS containing 15 mM in sodium azide, pH 7.4
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About B2M / Beta 2 Microglobulin

P61769 NM_004048 NP_004039.1

B2M Antibody, Beta 2 Microglobulin Antibody, Beta-2-microglobulin Antibody, Beta-2-microglobin Antibody

B2M / Beta 2 Microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.


Anti-B2M / Beta 2 Microglobulin antibody IHC of human kidney, glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8113 dilution 1:50.

Requested From: United States
Date Requested: 10/22/2016

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