Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
B2M / Beta 2 Microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
Anti-B2M / Beta 2 Microglobulin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7757 dilution 10 ug/ml.
Immunofluorescence of monoclonal antibody to B2M on HeLa cell. [antibody concentration 10 ug/ml]
B2M monoclonal antibody, clone 3F9-2C2 Western blot of B2M expression in U-2 OS.
Western blot of B2M expression in transfected 293T cell line by B2M monoclonal antibody, clone 3F9-2C2.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged B2M is approximately 0.03 ng/ml as a capture antibody.
Western blot of B2M over-expressed 293 cell line, cotransfected with B2M Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with B2M monoclonal antibody, clone 3F9-2C2. GAPDH ( 36.1 kD ) used as specificity a.