Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Specificity and Use
B cell antibody was raised against the human B-lymphoblastoid cell line, HRIK.
Detects a glycoprotein of 105kD found on circulating B-lymphocytes. Reacts with HRIK and Raji cell lines. Clinical Expression: B cell prolymphocytic leukemia (B-PLL): strongly positive; Hairy cell leukemia (HCL): strongly positive; Hairy cell leukemia variant (HCL-V): strongly positive; Splenic lymphoma with villous lymphocytes (SLVL): positive; B cell chronic lymphocytic leukemia (B-CLL): negative to weakly positive. No reaction with granulocytes, monocytes, platelets, erythrocytes, T lymphocytes or null cells.
Suitable for use in Flow Cytometry. Flow Cytometry: Neat. Use 10 ul per 100 ul of whole blood, or 1x1E6 peripheral blood mononuclear cells (PBMC) in 100 ul buffer. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Supplied in 1.0 ml 0.1 M Tris-HCl, 0.5 M sodium chloride, 10 mM glycine, pH 8.0, 0.2% BSA, 0.1% sodium azide.