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Anti-ATXN3 / JOS Antibody (aa1-245) LS-C186388

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.73 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

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Rabbit Polyclonal (IgG) to Human ATXN3 / JOS
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Human ATXN3 / JOS
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Rat, Bovine, Pig (at least 90% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (1:100 - 1:1000), Western blot (1:500 - 1:3000)

Specificity and Use

ATXN3 / JOS antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 245 of Ataxin 3 (SwissProt P54252). Percent identity by BLAST analysis: Human (100%); Pig (99%); Bovine (98%); Rat (96%).
aa1-245
Human ATXN3 / JOS
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About ATXN3 / JOS

P54252 NM_004993 NP_004984.2

ATXN3 Antibody, ATX3 Antibody, Ataxin 3 Antibody, Ataxin 3 variant h Antibody, AT3 Antibody, Ataxin 3 variant m Antibody, Ataxin 3 variant ref Antibody, Ataxin-3 Antibody, Olivopontocerebellar ataxia 3 Antibody, SCA3 Antibody, MJD1 Antibody, JOS Antibody, Josephin Antibody, MJD Antibody

Deubiquitinating enzyme involved in protein homeostasis maintenance, transcription, cytoskeleton regulation, myogenesis and degradation of misfolded chaperone substrates. Binds long polyubiquitin chains and trims them, while it has weak or no activity against chains of 4 or less ubiquitins.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded H661 xenograft using Ataxin 3 antibody at 1:500 dilution.

Western blot

Western blot
Sample (50 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:500.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: U87-MG. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:1000.
 

Requested From: United States
Date Requested: 5/1/2016

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