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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ATG4A Antibody (aa251‑300) LS‑C116218
ATG4A antibody LS-C116218 is an unconjugated rabbit polyclonal antibody to ATG4A from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C116218-50
50 µg
Unavailable

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Product Description

ATG4A antibody LS-C116218 is an unconjugated rabbit polyclonal antibody to ATG4A from human, mouse, rat and other species. Validated for WB.
About ATG4A
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms. Q8WYN0 NM_052936 Q8WYN0

ATG4A Antibody, AUTL2 Antibody, Autophagin-2 Antibody, APG4A Antibody, Autophagin 2 Antibody, Cysteine protease ATG4A Antibody, HAPG4A Antibody, APG4 autophagy 4 homolog A Antibody


Specifications

Target
Human ATG4A
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
ATG4A antibody was raised against synthetic peptide located between aa251-300 of human ATG4A (Q8WYN0, NP_443168). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig (100%); Opossum, Platypus, Xenopus (92%); Turkey, Zebra finch, Chicken (84%).
Blocking Peptide
LS-E15733 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116218. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa251-300
Specificity
Human ATG4A
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

ATG4A antibody LS-C116218 Western blot of Fetal Spleen lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATG4A antibody LS-C116218 Western blot of Fetal Spleen lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ATG4A antibody LS-C116218 Western blot of Fetal Spleen lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATG4A antibody LS-C116218 Western blot of Fetal Spleen lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ATG4A antibody LS-C116218 Western blot of Fetal Spleen lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATG4A antibody LS-C116218 Western blot of Fetal Spleen lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ATG4A antibody LS-C116218 Western blot of Fetal Spleen lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATG4A antibody LS-C116218 Western blot of Fetal Spleen lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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