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Anti-ATG4A Antibody (aa251-300) LS-C116218

Catalog Size Price
LS-C116218-50 50 µg Unavailable

Related Products

24 ATG4A Antibodies

Most Popular ATG4A Antibodies

Anti-ATG4A Antibody (aa81-130) LS-C119886
Rabbit Polyclonal (IgG) to Human ATG4A
Human, Mouse
IHC - Paraffin, Immunofluorescence, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ATG4A Antibody (C-Terminus) IHC-plus™ LS-B12105
Rabbit Polyclonal to Human ATG4A
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ATG4A Antibody (aa50-130) LS-C382180
Rabbit Polyclonal (IgG) to Human ATG4A
Human, Mouse
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-ATG4A Antibody LS-C402917
Rabbit Polyclonal (IgG) to Human ATG4A
Human
IHC, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ATG4A Antibody LS-C406149
Rabbit Polyclonal (IgG) to Human ATG4A
Human
IHC, Western blot, ELISA
Unconjugated

100% Guaranteed
Rabbit Polyclonal to Human ATG4A
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Zebrafish
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human ATG4A
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Xenopus (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

ATG4A antibody was raised against synthetic peptide located between aa251-300 of human ATG4A (Q8WYN0, NP_443168). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig (100%); Opossum, Platypus, Xenopus (92%); Turkey, Zebra finch, Chicken (84%).
LS-E15733 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116218. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa251-300
Human ATG4A
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About ATG4A

Q8WYN0 NM_052936 Q8WYN0

ATG4A Antibody, AUTL2 Antibody, Autophagin-2 Antibody, APG4A Antibody, Autophagin 2 Antibody, Cysteine protease ATG4A Antibody, HAPG4A Antibody, APG4 autophagy 4 homolog A Antibody

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Western blot

Western blot
ATG4A antibody LS-C116218 Western blot of Fetal Spleen lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 6/27/2017

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