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Anti-Aspartate Aminotransferase Antibody (aa157-167) IHC-plus™ LS-B2405

Note: This antibody replaces LS-C54927

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Aspartate Aminotransferase Antibodies

Anti-Aspartate Aminotransferase Antibody IHC-plus™ LS-B24
Sheep Polyclonal to Pig Aspartate Aminotransferase
Pig, Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-Aspartate Aminotransferase Antibody IHC-plus™ LS-B12294
Rabbit Polyclonal (IgG) to Human Aspartate Aminotransferase
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry - Paraffin Image
Anti-Aspartate Aminotransferase Antibody (aa157-167) IHC-plus™ LS-B2405
Goat Polyclonal to Human Aspartate Aminotransferase
Human, Rat, Hamster
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human Aspartate Aminotransferase
Human, Rat, Hamster
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human Aspartate Aminotransferase
Goat
Human, Rat, Hamster (tested or 100% immunogen sequence identity)
Monkey, Goat, Pig (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 - 5 µg/ml)
  • Western blot (0.01 - 0.1 µg/ml)
  • ELISA (1:4000)

Specificity and Use

Aspartate Aminotransferase antibody was raised against synthetic peptide C-RSYRYWDAEKR from an internal region of human GOT1 (NP_002070.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Rat, Hamster, Elephant (100%); Marmoset, Goat, Pig, Lizard (91%); Mouse, Panda, Bat, Bovine, Horse, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Pufferfish, Seq squirt (82%).
LS-E26542 - Lyophilized - 100 µg - $145.00
aa157-167
Human GOT1.
Immunohistochemistry: LS-B2405 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2405 was determined to be 2.5-5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About Aspartate Aminotransferase

P17174 NM_002079 NP_002070.1

GOT1 Antibody, Aspartate Aminotransferase Antibody, ASTQTL1 Antibody, Growth-inhibiting protein 18 Antibody, Transaminase A Antibody, GIG18 Antibody

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide.

Immunohistochemistry

Immunohistochemistry
Anti-GOT1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2405 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-GOT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2405 concentration 5 ug/ml.

Western blot

Western blot
Antibody (0.01 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 12/8/2016

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