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Anti-ARAF / ARAF1 / A-RAF Antibody (aa254-303) LS-C81879

Catalog Size Price
LS-C81879-50 50 µg Unavailable

Most Popular ARAF / ARAF1 / A-RAF Antibodies

Anti-ARAF / ARAF1 / A-RAF Antibody (aa276-325) IHC-plus™ LS-B8371
Rabbit Polyclonal (IgG) to Human ARAF / ARAF1 / A-RAF
Human, Mouse, Rat
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-ARAF / A RAF antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8371 dilution 10 ug/ml.
Anti-ARAF / ARAF1 / A-RAF Antibody (aa371-554) LS-C185401
Rabbit Polyclonal (IgG) to Human ARAF / ARAF1 / A-RAF
Human
IHC, IHC - Paraffin, ICC, Western blot
Unconjugated
IHC of paraffin-embedded Cal27 xenograft using ARAF antibody at 1:500 dilution.
Anti-ARAF / ARAF1 / A-RAF Antibody (phospho-Tyr302) LS-C198640
Rabbit Polyclonal (IgG) to Human ARAF / ARAF1 / A-RAF
Human, Mouse, Rat
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
IHC of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The sample on the right was incubated with synthetic peptide.
Anti-ARAF / ARAF1 / A-RAF Antibody (aa145-365, clone 2G9) LS-C338826
Mouse Monoclonal [clone 2G9] (IgG2a) to Human ARAF / ARAF1 / A-RAF
Human
IHC, IHC - Paraffin, Western blot
Unconjugated
IHC of paraffin-embedded Human prostate tissue using anti-ARAF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Anti-ARAF / ARAF1 / A-RAF Antibody (phospho-Tyr302) LS-C381494
Rabbit Polyclonal (IgG) to Human ARAF / ARAF1 / A-RAF
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated

100% Guaranteed
Rabbit Polyclonal (IgG) to Human ARAF / ARAF1 / A-RAF
Human, Mouse, Rat, Bat, Bovine, Horse, Pig, Sheep
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human ARAF / ARAF1 / A-RAF
Rabbit
Human, Mouse, Rat, Bat, Bovine, Horse, Pig, Sheep (tested or 100% immunogen sequence identity)
Monkey, Dog, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

Synthetic peptide located between aa254-303 of human ARAF (P10398, NP_001645). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Bovine, Bat, Horse, Pig (100%); Marmoset, Dog, Rabbit (92%); Elephant (85%).
LS-E19664 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C81879. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa254-303
Human ARAF
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.

About ARAF / ARAF1 / A-RAF

P10398 NM_001654 NP_001645.1

ARAF Antibody, A RAF Antibody, A-RAF Antibody, A-raf-1 Antibody, PKS Antibody, PKS2 Antibody, Proto-oncogene A-Raf Antibody, Proto-oncogene A-Raf-1 Antibody, Ras-binding protein DA-Raf Antibody, Oncogene ARAF1 Antibody, ARAF1 Antibody, Proto-oncogene Pks Antibody, RAFA1 Antibody

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.

Western blot

Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 5/22/2018

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