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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B8371-100 100 µl (1 mg/ml) $505 
ARAF / ARAF1 / A-RAF Antibody - Anti-ARAF / A RAF antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
ARAF / ARAF1 / A-RAF Antibody - Western blot analysis of lysates from HeLa cells, treated with PMA 125ng/ml 30', using A-RAF Antibody. The lane on the right is blocked with the synthesized peptide.
ARAF / ARAF1 / A-RAF Antibody - Anti-ARAF / A RAF antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
ARAF / ARAF1 / A-RAF Antibody - Western blot analysis of lysates from HeLa cells, treated with PMA 125ng/ml 30', using A-RAF Antibody. The lane on the right is blocked with the synthesized peptide.
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IHCPlus™ Polyclonal Rabbit anti‑Human ARAF / ARAF1 / A‑RAF Antibody (aa276‑325, IHC, WB) LS‑B8371

IHCPlus™ Polyclonal Rabbit anti‑Human ARAF / ARAF1 / A‑RAF Antibody (aa276‑325, IHC, WB) LS‑B8371

Note: This antibody replaces LS-C117794
Antibody:
Rabbit Polyclonal to Human ARAF / ARAF1 / A-RAF
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$505 
LS-B8371-100
100 µl
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human ARAF / ARAF1 / A-RAF
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
A-RAF antibody LS-B8371 is an unconjugated rabbit polyclonal antibody to A-RAF (ARAF / ARAF1) (aa276-325) from human. It is reactive with human, mouse and rat. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ARAF / ARAF1 / A-RAF
Synonyms
ARAF | A RAF | A-RAF | A-raf-1 | PKS | PKS2 | Proto-oncogene A-Raf | Proto-oncogene A-Raf-1 | Ras-binding protein DA-Raf | Oncogene ARAF1 | ARAF1 | Proto-oncogene Pks | RAFA1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human A-RAF. (AA Range: 276-325)
Epitope
aa276-325
Specificity
A-RAF (Ab-301/302) Antibody detects endogenous levels of total A-RAF protein.
Usage
Western Blot: Predicted MW 67 kDa
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ARAF / ARAF1 / A-RAF
P10398 NM_001654 NP_001645.1

Validation

ARAF / ARAF1 / A-RAF Antibody - Anti-ARAF / A RAF antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Anti-ARAF / A RAF antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Anti-ARAF / A RAF antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.

LSBio Ratings

IHC-plus™ ARAF / ARAF1 / A-RAF Antibody (aa276-325) for IHC, WB/Western LS-B8371 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 5/28/2020