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Anti-ALDOA / Aldolase A Antibody IHC-plus™ LS-B1461

Note: This antibody replaces LS-C19090, LS-C34938, LS-C34937, LS-C142423

Ordering

Wt. Vol. Conc. Price
500 µg - 10 mg/ml $395
Inquire for larger quantities

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Most Popular ALDOA / Aldolase A Antibodies

Anti-ALDOA / Aldolase A Antibody IHC-plus™ LS-B1461
Goat Polyclonal to Rabbit ALDOA / Aldolase A
Rabbit, Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ALDOA / Aldolase A Antibody (aa303-331) LS-C98593
Rabbit Polyclonal to Human ALDOA / Aldolase A
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ALDOA / Aldolase A Antibody (clone 2E6) IHC-plus™ LS-B6377
Mouse Monoclonal [clone 2E6] (IgG2a,k) to Human ALDOA / Aldolase A
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Goat Polyclonal to Rabbit ALDOA / Aldolase A
Rabbit, Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Rabbit ALDOA / Aldolase A
Goat
Rabbit, Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ion exchange chromatography
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • Western blot (1:1000)
  • Immunoprecipitation
  • ELISA

Specificity and Use

ALDOA / Aldolase A antibody was raised against purified aldolase from rabbit muscle
Aldolase [Rabbit Muscle].
Immunohistochemistry: LS-B1461 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1461 was determined to be 5 ug/ml.

Packaging

0.02M PBS, pH 7.2, 0.01% sodium azide
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (1)

1
Proteomic profiling of osteosarcoma cells identifies ALDOA and SULT1A3 as negative survival markers of human osteosarcoma. Chen X, Yang TT, Zhou Y, Wang W, Qiu XC, Gao J, Li CX, Long H, Ma BA, Ma Q, Zhang XZ, Yang LJ, Fan QY. Molecular carcinogenesis. 2014 Feb;53:138-44. [Full Text Article] [PubMed:22949271]

About ALDOA / Aldolase A

P04075 NM_000034 NP_000025.1

ALDOA Antibody, ALDA Antibody, Aldolase Antibody, GSD12 Antibody, Muscle-type aldolase Antibody, Lung cancer antigen NY-LU-1 Antibody

ALDOA / Aldolase A, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle.

Immunohistochemistry

Immunohistochemistry
Anti-Aldolase antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1461 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-Aldolase antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1461 concentration 5 ug/ml.

Western blot

Western blot
Anti-Aldolase Antibody - Western Blot. IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% Tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4° C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kD consistent with human aldolase.

Western blot

Western blot
Anti-Aldolase (Rabbit Muscle) was used at a 1:1000 dilution to detect human aldolase by Western blot. This antibody clearly detects a band at ~41 kDa consistent with human aldolase.

Requested From: United States
Date Requested: 12/5/2016

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