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Anti-AKT1 Antibody (phospho-Ser473, clone 104A282) LS-C581

Note: This antibody replaces LS-C63895, LS-C3588

Ordering

Wt. Vol. Conc. Price
100 µg 200 µl 0.5 mg/ml $335
Inquire for larger quantities

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Mouse Monoclonal [clone 104A282] (IgG) to Human AKT1
Human, Mouse
Western blot, Immunoprecipitation
Unconjugated

Details

Human AKT1
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Monoclonal [104A282]
Protein G purified

Applications

Western blot (2 µg/ml), Immunoprecipitation (1 µg/ml)

Specificity and Use

AKT1 antibody was raised against a synthetic peptide containing phospho serines at amino acid residues 473 of human AKT, Accession no. ref|NP_005154.2|. The sequence in this region is identical in human, mouse, chicken, and frog.
pSer473
Clone 104A282 detects specifically the Ser 473 Phosphorylated form of AKT.

Packaging

PBS, 0.2% gelatin, 0.05% sodium azide.
Long term: -70°C; Short term: +4°C
For research use only.

About AKT1

P31749 NM_005163 NP_005154.2

AKT1 Antibody, AKT Antibody, AKT-1 Antibody, C-AKT Antibody, Pan-AKT Antibody, PKB alpha Antibody, PKBalpha Antibody, Proto-oncogene c-Akt Antibody, Protein kinase B Antibody, Rac protein kinase alpha Antibody, PRKBA Antibody, Protein kinase B alpha Antibody, RAC-PK-alpha Antibody, PKB Antibody, PKB-ALPHA Antibody, RAC Antibody, RAC-ALPHA Antibody

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported.

Western blot

Western blot
Western blot of AKT1 using antibody at 2 ug/ml dilution against 10 ug/lane of untreated (lane 1) and PDGF treated (lane 2) NIH-3T3 lysate.
 

Requested From: United States
Date Requested: 9/30/2016

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