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AKT1

v-akt murine thymoma viral oncogene homolog 1

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI3P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. May be involved in the regulation of the placental development. Phosphorylates STK4/MST1 at 'Thr-120' and 'Thr-387' leading to inhibition of its: kinase activity, nuclear translocation, autophosphorylation and ability to phosphorylate FOXO3. Phosphorylates STK3/MST2 at 'Thr-117' and 'Thr-384' leading to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation. Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation. Phosphorylates RAF1 at 'Ser-259' and negatively regulates its activity. Phosphorylation of BAD stimulates its pro-apoptotic activity. Phosphorylates KAT6A at 'Thr-369' and this phosphorylation inhibits the interaction of KAT6A with PML and negatively regulates its acetylation activity towards p53/TP53.AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

Gene Name: v-akt murine thymoma viral oncogene homolog 1
Family/Subfamily: Protein Kinase , RAC/AKT
Synonyms: AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA
Target Sequences: NM_005163 NP_005154.2 P31749

Publications (3)

1
Activation of phosphatidylinositol 3-kinase by membrane localization of p110alpha predisposes mammary glands to neoplastic transformation. Renner O, Blanco-Aparicio C, Grassow M, Caamero M, Leal JF, Carnero A. Cancer research. 2008 68:9643-53. (IHC) [PubMed:19047141]
2
GGAP2/PIKE-a directly activates both the Akt and nuclear factor-kappaB pathways and promotes prostate cancer progression. Cai Y, Wang J, Li R, Ayala G, Ittmann M, Liu M. Cancer research. 2009 69:819-27. [PubMed:19176382] [PMC:PMC2668918] Related Antibodies: LS-C98936.
3
Ephrin-a4 is involved in retinal neovascularization by regulating the VEGF signaling pathway. Du W, Yu W, Huang L, Zhao M, Li X. Investigative ophthalmology & visual science. 2012 53:1990-8. [PubMed:22408005]

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AKT1 Antibody - AKT pS473 Monoclonal Antibody - Immunohistochemistry. anti-AKT pS473 monoclonal antibody in immunohistochemistry shows detection of phosphorylated AKT pS473 in human prostate tissue. The antibody was used at 20 ug/mL. The staining is much stronger than the weak basal level of phosphorylation in normal prostate tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required.
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AKT1 Mouse anti-Human Monoclonal (pSer473) (17F6.B11) Antibody
Mouse, Rat, Human, Monkey
ELISA, Flo, IF, IHC, IP, WB
Unconjugated
100 µg/$574
AKT1 Antibody - Anti-AKT1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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AKT1 Rabbit anti-Human Polyclonal (aa121-170) Antibody
Frog, Baboon, Human
IHC, IHC-P, WB
Unconjugated
100 µl/$485
AKT1 ELISA Kit
Select
Cell-Based Phosphorylation
96-Well Microplate
Mouse, Human, Rat
Colorimetric - 450nm (TMB)
Adherent Cell Cultures
1 Plate/$668
AKT1 Antibody - Western blot of hAKT1-D453 in Y79 cell line lysates (35 ug/lane). AKT1 (arrow) was detected using the purified antibody.
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AKT1 Rabbit anti-Human Polyclonal (aa438-468) Antibody
Human
Flo, WB
Unconjugated
400 µl/$393
AKT1 Antibody - Anti-AKT1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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AKT1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IF, IHC, IHC-P, IP, WB
Unconjugated
0.05 mg/$460
AKT1 Antibody - Anti-AKT1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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AKT1 Rabbit anti-Human Polyclonal (pThr308) Antibody
Mouse, Rat, Human
ELISA, Flo, IHC, IHC-P, WB
Unconjugated
50 µg/$505
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AKT1 Rabbit anti-Human Polyclonal (pThr308) Antibody
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
50 µg/$489
AKT1 Antibody - AKT1 Antibody (C-term T450) immunohistochemistry of formalin-fixed and paraffin-embedded human breast carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
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AKT1 Rabbit anti-Human Polyclonal (aa428-457) Antibody
Mouse, Human
Flo, IF, IHC, IHC-P, WB
Unconjugated
400 µl/$393
AKT1 Antibody - Immunohistochemistry of mouse anti AKT phospho S473 biotin conjugated at 40X (left) with negative control (right) Tissue: prostate Fixation: FFPE buffered formalin 10% conc Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 20 g/mL for 1 h at RT Secondary antibody: Streptavidin Conj. HRP 10 ug/ml Localization: nuclear and occasionally cytoplasmic Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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AKT1 Mouse anti-Human Monoclonal (pSer473) (Biotin) (17F6.B11) Antibody
Mouse, Rat, Human, Monkey
ELISA, Flo, IF, IHC, IP, WB
Biotin Conjugated
50 µg/$472
AKT1 Antibody - Anti-AKT Monoclonal Antibody - Western Blot. anti-AKT Monoclonal Antibody in western blot shows detection of AKT (indicated by arrowhead at ~56 kD) on unstimulated (lane 1) and PDGF stimulated NIH/3T3 cell lysates (lane 2). Each lane contained approximately 10 ug of lysate. All samples were loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 5% BLOTTO (B501-0500 in TBS for 2h at RT. The membrane was probed with Anti-AKT antibody at a 1:1000 dilution in TBS with 5% BLOTTO, overnight at 4° C. For detection HRP conjugated Gt-a-Mouse IgG (p/n LS-C60680) was used at a 1:40000 dilution for 1 h at 4° C with FemtoMax enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.).
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AKT1 Mouse anti-Human Monoclonal (Internal) (14E5.16C8.25F6) Antibody
Mouse, Rat, Chimpanzee, Human
ELISA, Flo, IHC, WB
Unconjugated
100 µg/$574
AKT1 Antibody - Immunohistochemistry of Mouse anti-AKT pT308 antibody. Tissue: human brain cerebellum tissue (40X). Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT pT308antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: staining of Purkinje neurons and cell processes in the cerebellum, cytosolic as well as occasionally nuclear. Staining: AKT pT308 as precipitated red signal with hematoxylin purple nuclear counterstain.
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AKT1 Mouse anti-Human Monoclonal (pThr308) (18F3.H11) Antibody
Mouse, Rat, Human, Monkey
ELISA, Flo, IHC, IP, WB
Unconjugated
25 µl/$304
AKT1 Antibody - IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AKT1 mouse monoclonal antibody.
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AKT1 Mouse anti-Human Monoclonal (OTI4E10) Antibody
Mouse, Dog, Rat, Human, Monkey
Flo, IF, IHC, IHC-P, WB
Unconjugated
100 µl/$434
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AKT1 Rabbit anti-Human Polyclonal (pThr450) (PE) Antibody
Human
DB, ELISA
PE Conjugated
200 µl/$952
AKT1 Antibody - Flow Cytometry of Mouse anti-AKT1 antibody. Cells: LNCap Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT1 antibody at 1.0 µg/mL for 20 min at 4°C.
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AKT1 Mouse anti-Human Monoclonal (Internal) (APC) (14E5.A2.B2.H9) Antibody
Mouse, Rat, Human
DB, ELISA, Flo, IF, IHC, WB
APC Conjugated
50 µg/$582
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AKT1 Rabbit anti-Human Polyclonal (aa428-457) (Azide-free) (HRP) Antibody
Mouse, Human
ELISA, Flo, ICC, IF, IHC, WB
HRP Conjugated, Azide-free
200 µl/$952
AKT1 Antibody - Phospho-Akt(Ser473) antibody for IHC in human brain tissue
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AKT1 Rabbit anti-Human Polyclonal (pSer473) Antibody
Mouse, Rat, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
AKT1 Antibody - Immunohistochemistry of paraffin-embedded mouse lung tissue.
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AKT1 Rabbit anti-Human Monoclonal (pSer473) Antibody
ICC, IF, IHC, WB
Unconjugated
20 µl/$299; 50 µl/$341; 100 µl/$431; 200 µl/$539
AKT1 Antibody - Western Blot - Anti-AKT Antibody. Anti-AKT Antibody in western blot showing detection of endogenous protein in whole cell extracts from H2O2 treated MCF7 cells (lane 2). Recombinant AKT is detected in lane 3. Lanes 1 and 4 contain wcl from HeLa and A431 cells, respectively. Specific band staining is completely blocked when antibody is pre-incubated with the immunizing peptide (data not shown). The band at ~55-60 kD, indicated by the arrowhead, corresponds to the expected molecular weight of AKT. AKT antibody was diluted 1:300 in 1% BLOTTO and reacted with the membrane overnight at 4° C. IRDYE800 conjugated Dnky-a-Sheep IgG was used at a 1:10000 dilution for 45 min at room temperature.
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AKT1 Sheep anti-Human Polyclonal (Internal) Antibody
Mouse, Rat, Human
ELISA, Flo, IF, IHC, WB
Unconjugated
100 µg/$567
AKT1 Antibody - Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Akt Antibody. The picture on the right is blocked with the synthesized peptide.
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AKT1 Rabbit anti-Human Polyclonal (aa292-341) Antibody
Mouse, Rat, Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
AKT1 Antibody - Anti-AKT pT308 Monoclonal Antibody - Immunohistochemistry. anti-AKT pT308 monoclonal antibody in immunohistochemistry shows detection of phosphorylated AKT pT308 in human brain cerebellum tissue (40X). Anti-AKTpT308 antibody was used at dilution of 20 ug/mL. The image shows strong staining of Purkinje neurons and cell processes in the cerebellum. Staining was both cytosolic as well as occasionally nuclear, and the Anti-AKT pT308 antibody showed minimal background staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.
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AKT1 Mouse anti-Human Monoclonal (pThr308) (18F3.H11) Antibody
Mouse, Rat, Human, Monkey
ELISA, Flo, IHC, IP, WB
Unconjugated
100 µg/$574
AKT1 ELISA Kit
Select
Sandwich
96-Well Strip Plate
Human
0.78 - 50 ng/ml
Colorimetric - 450nm (TMB)
1 Plate/$685
AKT1 Protein - Recombinant Human pan-AKT protein (His Tag)
Select
E. coli
His, N-terminus
55.7kDa
20 µg/$270; 100 µg/$390
AKT1 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
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AKT1 Rabbit anti-Human Polyclonal (aa95-144) Antibody
Mouse, Rat, Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
100 µl/$515
AKT1 Antibody - Immunohistochemical analysis of AKT staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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AKT1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Mouse, Sheep, Bovine, Rat, Pig, Human
IHC, IHC-P, IP, WB
Unconjugated
100 µl/$299
AKT1 Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Akt (Phospho-Ser124) Antibody. The picture on the right is blocked with the phospho peptide.
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AKT1 Rabbit anti-Human Polyclonal (pSer124) Antibody
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).