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AKT1

v-akt murine thymoma viral oncogene homolog 1

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI3P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. May be involved in the regulation of the placental development. Phosphorylates STK4/MST1 at 'Thr-120' and 'Thr-387' leading to inhibition of its: kinase activity, nuclear translocation, autophosphorylation and ability to phosphorylate FOXO3. Phosphorylates STK3/MST2 at 'Thr-117' and 'Thr-384' leading to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation. Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation. Phosphorylates RAF1 at 'Ser-259' and negatively regulates its activity. Phosphorylation of BAD stimulates its pro-apoptotic activity. Phosphorylates KAT6A at 'Thr-369' and this phosphorylation inhibits the interaction of KAT6A with PML and negatively regulates its acetylation activity towards p53/TP53.AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

Gene Name: v-akt murine thymoma viral oncogene homolog 1
Family/Subfamily: Protein Kinase , RAC/AKT
Synonyms: AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA
Target Sequences: NM_005163 NP_005154.2 P31749

Publications (3)

1
Activation of phosphatidylinositol 3-kinase by membrane localization of p110alpha predisposes mammary glands to neoplastic transformation. Renner O, Blanco-Aparicio C, Grassow M, Caamero M, Leal JF, Carnero A. Cancer research. 2008 68:9643-53. (IHC) [PubMed:19047141]
2
GGAP2/PIKE-a directly activates both the Akt and nuclear factor-kappaB pathways and promotes prostate cancer progression. Cai Y, Wang J, Li R, Ayala G, Ittmann M, Liu M. Cancer research. 2009 69:819-27. [PubMed:19176382] [PMC:PMC2668918] Related Antibodies: LS-C98936.
3
Ephrin-a4 is involved in retinal neovascularization by regulating the VEGF signaling pathway. Du W, Yu W, Huang L, Zhao M, Li X. Investigative ophthalmology & visual science. 2012 53:1990-8. [PubMed:22408005]

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AKT1 (406)
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Publications
No (405)
Yes (1)
Showing 1-25 of 406 results

AKT1 Antibody (phospho-Ser473) IHC-plus™ LS-B1183
Rabbit Polyclonal to Human AKT1
Mouse, Rat, Human
ELISA ICC IF IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-AKT1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AKT1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

AKT1 Antibody (aa121-170) IHC-plus™ LS-B1510
Rabbit Polyclonal (IgG) to Human AKT1
Xenopus, Chicken, Sheep, Bovine, Mouse, Goat, Dog, Guinea pig, Rat, Hamster, Horse, Human, Monkey
IHC IHC-P WB
Unconjugated
100µl 0.5mg/ml  $485.00
Anti-AKT1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-AKT1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

AKT1 Antibody (phospho-Thr308) LS-C122634
Rabbit Polyclonal (IgG) to Human AKT1
Mouse, Rat, Human
IF IHC WB
Unconjugated
50µg 1mg/ml  $435.00

AKT1 Antibody (aa438-468) LS-C98936
Publications (1)
Publications (1)
Rabbit Polyclonal to Human AKT1
Human
Flo WB
Unconjugated
400µl  $305.00
Western blot of hAKT1-D453 in Y79 cell line lysates (35 ug/lane). AKT1 (arrow) was detected using the purified antibody.
Western blot of AKT1 (arrow) using rabbit polyclonal hAKT1-D453. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT1 gene (Lane 2) (Origene Technologies).

AKT1 Antibody (phospho-Thr308) IHC-plus™ LS-B1190
Rabbit Polyclonal to Human AKT1
Mouse, Rat, Human
ELISA IHC IHC-P WB
Unconjugated
50µg 0.97mg/ml  $425.00
Anti-AKT1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Western blot of Rabbit anti-Akt phospho T308 antibody. Lane 1: GST tagged AKT1 un-active recombinant protein. Lane 2: GST tagged AKT1 active recombinant protein. Load: 50 ng per lane. Primary antibody: Akt phospho T308 antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:20000 for 30 min at RT. Block: MB-070 for 30 min at RT. Other band(s): none.

AKT1 Antibody (C-Terminus, clone 18F10.E5) LS-C154001
Mouse Monoclonal [clone 18F10.E5] (IgG) to Human AKT1
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
100µg 1mg/ml  $425.00
Anti-AKT Monoclonal Antibody - Western Blot. anti-AKT Monoclonal Antibody in western blot shows detection of AKT (indicated by arrowhead at ~56 kD) on unstimulated (lane 1) and PDGF stimulated NIH/3T3 cell lysates (lane 2). Each lane contained approximately 10 ug of lysate. All samples were loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 5% BLOTTO (B501-0500 in TBS for 2h at RT. The membrane was probed with Anti-AKT antibody at a 1:1000 dilution in TBS with 5% BLOTTO, overnight at 4° C. For detection HRP conjugated Gt-a-Mouse IgG (p/n LS-C60680) was used at a 1:40000 dilution for 1 h at 4° C with FemtoMax enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.).

AKT1 Antibody (clone 4E10) LS-C173711
Mouse Monoclonal [clone 4E10] (IgG1) to Human AKT1
Mouse, Dog, Rat, Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated
100µl 0.68mg/ml  $335.00
IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-AKT1 mouse monoclonal antibody.

AKT1 Antibody (aa428-457) LS-C156225
Rabbit Polyclonal to Human AKT1
Mouse, Human
Flo IF IHC IHC-P WB
Unconjugated
400µl  $305.00
AKT1 Antibody (C-term T450) immunohistochemistry of formalin-fixed and paraffin-embedded human breast carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Confocal immunofluorescence of AKT1 Antibody (C-term T450) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

AKT1 Antibody (phospho-Ser473, clone 17F6.B11) LS-C153997
Mouse Monoclonal [clone 17F6.B11] (IgG1) to Human AKT1
Mouse, Rat, Human, Monkey
ELISA IF IHC IHC-P WB
Unconjugated
100µg 1mg/ml  $425.00
AKT pS473 Monoclonal Antibody - Immunohistochemistry. anti-AKT pS473 monoclonal antibody in immunohistochemistry shows detection of phosphorylated AKT pS473 in human prostate tissue. The antibody was used at 20 ug/mL. The staining is much stronger than the weak basal level of phosphorylation in normal prostate tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required.
AKT pS473 Monoclonal Antibody - Immunofluorescence Microscopy. High resolution STED immunofluorescence nanoscopy showing detection of phosphorylated AKT. anti-AKT pS473 monoclonal antibody Anti-AKT pS473 (MOUSE) Monoclonal Antibody - 200-301-268 was used at 10 ug/mL for 1 h at RT to stain A431 cells after serum deprivation for 12 h. Cells were fixed in 4% paraformaldehyde for 5 min and after washes blocked with 10% NGS/0.2% Triton X-100 for 30 min. Phosphorylated AKT (red) is observed to be localized in the cytoplasm and also organized at the periphery of the cell. Sequential staining at 1.4 ug/mL for 1 h at RT with rabbit anti-a tubulin (cyan) (LS-B722) is also shown. The merge images (A) and at high magnification (B) show phosphorylated AKT colocalized with the distal microtubules. High magnification images (D, E and F) show phosphorylated AKT appears to be colocalized to microtubules. Images were taken with a 100x objective on a Leica TCS STED (Stimulated Emission and Depletion), Leica Microsystems, Inc. Exton, PA, USA. Atto 647N anti-Mouse IgG (ATTO TEC GmbH), and DyLight488 anti-Rabbit IgG (p/n were used at 1.0 ug/mL for 1h at RT for indirect detection. Prolong antifade reagent (Invitrogen) was used to mount slides. Personal Communication, Myriam Gastard, Leica Microsystems Inc, Exton, PA (USA).

AKT1 Antibody LS-C497717
Rabbit Monoclonal to Human AKT1
Mouse, Rat, Human
Flo IF IHC IP WB
Unconjugated
50µl  $295.00; 100µl  $385.00; 200µl  $560.00
Immunohistochemistry of paraffin-embedded mouse brain using AKT1 antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using AKT1 antibodyat dilution of 1:100 (40x lens).

AKT1 Antibody LS-C497716
Rabbit Monoclonal to Human AKT1
Mouse, Rat, Human
Flo IF IHC IP WB
Unconjugated
50µl  $295.00; 100µl  $385.00; 200µl  $560.00
Immunohistochemistry of paraffin-embedded mouse brain using Pan-AKT antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using Pan-AKT antibodyat dilution of 1:100 (40x lens).

AKT1 Antibody (phospho-Thr450, PE) LS-C252845
Rabbit Polyclonal (IgG) to Human AKT1
Human
DB ELISA
PE Conjugated
200µl 0.45mg/ml  $680.00

AKT1 Antibody (aa428-457, Azide-free, HRP) LS-C251144
Rabbit Polyclonal (IgG) to Human AKT1
Mouse, Human
ELISA Flo ICC IF IHC WB
HRP Conjugated, Azide-free
200µl 0.55mg/ml  $680.00

AKT1 Antibody (phospho-Ser473) LS-C497694
Rabbit Monoclonal (IgG) to Human AKT1
ICC IF IHC WB
Unconjugated
50µl  $295.00; 100µl  $385.00; 200µl  $560.00
Immunohistochemistry of paraffin-embedded mouse lung tissue.
Immunofluorescence analysis of NIH-3T3 cells.

AKT1 Antibody (aa292-341) LS-C117826
Rabbit Polyclonal (IgG) to Human AKT1
Mouse, Rat, Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Akt Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Akt Antibody. The picture on the right is blocked with the synthesized peptide.

AKT1 Antibody (phospho-Ser473, clone 17F6.B11, Biotin) LS-C153789
Mouse Monoclonal [clone 17F6.B11] (IgG) to Human AKT1
Mouse, Rat, Human, Monkey
ELISA IF IHC WB
Biotin Conjugated
50µg 0.5mg/ml  $365.00
Immunohistochemistry of mouse anti AKT phospho S473 biotin conjugated at 40X (left) with negative control (right) Tissue: prostate Fixation: FFPE buffered formalin 10% conc Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 20 g/mL for 1 h at RT Secondary antibody: Streptavidin Conj. HRP 10 ug/ml Localization: nuclear and occasionally cytoplasmic Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Mouse anti-Akt phospho S473 Biotin Conjugated antibody. Lane 1: GST tagged AKT1 active recombinant protein. Lane 2: none. Load: 25 ng per lane. Primary antibody: Akt phospho S473 Biotin Conjugated antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 79 kDa, 79 kDa for Akt phospho S473. Other band(s): none This image was taken for the unconjugated form of this product. Other forms have not been tested.

AKT1 Antibody (aa95-144) IHC-plus™ LS-B12420
Rabbit Polyclonal (IgG) to Human AKT1
Mouse, Rat, Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
100µl 1mg/ml  $505.00
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of HeLa cells, using Akt Antibody. The picture on the right is blocked with the synthesized peptide.

AKT1 Antibody (Thr308) IHC-plus™ LS-B1168
Rabbit Polyclonal (IgG) to Human AKT1
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-AKT1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Western blot of extracts from HepG2 and HeLa cells using Akt(Ab-308) antibody.

AKT1 Antibody IHC-plus™ LS-B1327
Rabbit Polyclonal (IgG) to Human AKT1
Mouse, Rat, Human
ELISA IF IHC IHC-P IP WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-AKT1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunofluorescence of Akt1 in Human Liver cells with Akt1 antibody at 20 ug/ml.

AKT1 Antibody LS-C122635
Rabbit Polyclonal (IgG) to Human AKT1
Human
IHC WB
Unconjugated
50µg 1mg/ml  $435.00

AKT1 Antibody LS-C658480
Rabbit Monoclonal (IgG) to Human AKT1
Human
IHC WB
Unconjugated
50µl  $445.00

AKT1 Antibody LS-C658479
Rabbit Monoclonal (IgG) to Human AKT1
Human
IHC WB
Unconjugated
50µl  $445.00

AKT1 Antibody (clone 4D6) LS-C173708
Mouse Monoclonal [clone 4D6] (IgG1) to Human AKT1
Mouse, Dog, Rat, Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated
100µl 0.73mg/ml  $345.00
IHC of paraffin-embedded Human Kidney tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-AKT1 mouse monoclonal antibody.

AKT1 Antibody (clone 3B4) LS-C173710
Mouse Monoclonal [clone 3B4] (IgG1) to Human AKT1
Mouse, Dog, Rat, Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated
100µl 0.89mg/ml  $335.00
IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-AKT1 mouse monoclonal antibody.

AKT1 Antibody (clone 3E3) LS-C173709
Mouse Monoclonal [clone 3E3] (IgG1) to Human AKT1
Mouse, Dog, Rat, Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated
100µl 0.58mg/ml  $335.00
IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-AKT1 mouse monoclonal antibody.
Showing 1-25 of 406 results

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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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