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Anti-AKT1 Antibody (aa2-480) LS-C125031

Catalog Size Price
LS-C125031-100 100 µg Unavailable

Related Products

383 AKT1 Antibodies

Most Popular AKT1 Antibodies

Anti-AKT1 Antibody (phospho-Ser473) IHC-plus™ LS-B1183
Rabbit Polyclonal to Human AKT1
Human, Mouse, Rat
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry of Rabbit Anti-Akt pS473 antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Akt pS473 antibody at 100 dilution for 1 h at RT. Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10000 for 45 min at RT. Localization: Akt pS473 is nuclear and occasionally cytoplasmic.
Anti-AKT1 Antibody (aa121-170) IHC-plus™ LS-B1510
Rabbit Polyclonal (IgG) to Human AKT1
Human, Monkey, Mouse, Rat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Sheep, Chicken, Xenopus
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-AKT1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1510 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-AKT1 Antibody (phospho-Thr308) LS-C122634
Rabbit Polyclonal (IgG) to Human AKT1
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated
Anti-AKT1 Antibody (C-Terminus, clone 18F10.E5) LS-C154001
Mouse Monoclonal [clone 18F10.E5] (IgG) to Human AKT1
Human, Mouse, Rat
IHC, Western blot, ELISA
Unconjugated
Anti-AKT Monoclonal Antibody - Western Blot. anti-AKT Monoclonal Antibody in western blot shows detection of AKT (indicated by arrowhead at ~56 kD) on unstimulated (lane 1) and PDGF stimulated NIH/3T3 cell lysates (lane 2). Each lane contained approximately 10 ug of lysate. All samples were loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 5% BLOTTO (B501-0500 in TBS for 2h at RT. The membrane was probed with Anti-AKT antibody at a 1:1000 dilution in TBS with 5% BLOTTO, overnight at 4° C. For detection HRP conjugated Gt-a-Mouse IgG (p/n LS-C60680) was used at a 1:40000 dilution for 1 h at 4° C with FemtoMax enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.).
Anti-AKT1 Antibody (phospho-Thr450, PE) LS-C252845
Rabbit Polyclonal (IgG) to Human AKT1
Human
ELISA, Dot Blot
PE Conjugated

100% Guaranteed
Mouse Monoclonal (IgG1) to Human AKT1
Human
ICC, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human AKT1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • ICC (8 - 25 µg/ml)
  • Western blot
  • Flow Cytometry (50 µg/ml)
  • ELISA

Specificity and Use

AKT1 antibody was raised against sf 21-derived recombinant human v-akt murine thymoma viral oncogene homolog 1 aa2-480.
aa2-480
Recognizes human Akt1. Does not detect rhAkt2 or rhAkt3.
Suitable for use in ELISA, Western Blot, Immunocytochemistry, Suitable for use in Flow cytometry: 50 ug/ml. This antibody was validated for flow cytometry using MCF-7 cells. For. intracellular staining to detect Akt1, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-5x10^5 cells in a total reaction volume not exceeding 20ulL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1% saponin prior to flow cytometric analysis. Immunocytochemistry: 8-25 ug/ml to detect Akt1 in fixed human peripheral blood mononuclear cells.

Packaging

Lyophilized from 0.2 um sterile-filtered solution, PBS, 5% trehalose
Sterile PBS
Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
For research use only.

About AKT1

P31749 NM_005163 NP_005154.2

AKT1 Antibody, AKT Antibody, AKT-1 Antibody, C-AKT Antibody, Pan-AKT Antibody, PKB alpha Antibody, PKBalpha Antibody, Proto-oncogene c-Akt Antibody, Protein kinase B Antibody, Rac protein kinase alpha Antibody, PRKBA Antibody, Protein kinase B alpha Antibody, RAC-PK-alpha Antibody, PKB Antibody, PKB-ALPHA Antibody, RAC Antibody, RAC-ALPHA Antibody

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported.

Requested From: 
Date Requested: 5/27/2018

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