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Anti-AKT1 + AKT2 + AKT3 Antibody (C-Terminus, Biotin) LS-C126659

Catalog Size Price
LS-C126659-100 100 µl Unavailable

Most Popular AKT1 + AKT2 + AKT3 Antibodies

Anti-AKT1 + AKT2 + AKT3 Antibody (C-Terminus) IHC-plus™ LS-B2844
Rabbit Polyclonal to Human AKT1 + AKT2 + AKT3
Human, Mouse, Rat, Chicken
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-AKT1/2/3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2844 dilution 1:100.
Anti-AKT1 + AKT2 + AKT3 Antibody (phospho-Ser473) LS-C117286
Rabbit Polyclonal (IgG) to Human AKT1 + AKT2 + AKT3
Human, Mouse, Rat
IHC, Western blot, ELISA
Unconjugated
Western blot of extracts from HeLa cells treated with heat shock, using Akt (Phospho-Ser473) Antibody. The lane on the left is treated with the synthesized peptide.
Anti-AKT1 + AKT2 + AKT3 Antibody (Internal) LS-C351801
Rabbit Polyclonal to Human AKT1 + AKT2 + AKT3
Human, Mouse, Rat, Bovine, Dog, Chicken, Zebrafish
IHC, IHC - Paraffin, Western blot
Unconjugated
Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-AKT1 + AKT2 + AKT3 Antibody (Internal) LS-C354521
Rabbit Polyclonal to Human AKT1 + AKT2 + AKT3
Human, Mouse, Rat, Bovine, Sheep
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of AKT staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. w
Anti-AKT1 + AKT2 + AKT3 Antibody (C-Terminus) LS-C354963
Rabbit Polyclonal (IgG) to Human AKT1 + AKT2 + AKT3
Human, Mouse
ICC, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
IF using AKT pan Antibody

100% Guaranteed
Mouse Monoclonal (IgG1,k) to Human AKT1 + AKT2 + AKT3
Human, Monkey, Mouse, Rat
Western blot, Immunoprecipitation, Flow Cytometry
Biotin Conjugated

Details

Human AKT1 + AKT2 + AKT3
Mouse
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Biotin
Ascites
Unmodified

Applications

  • Western blot (1:1000)
  • Immunoprecipitation (1:50)
  • Flow Cytometry (1:50)

Specificity and Use

Synthetic peptide corresponding to residues in the carboxy-terminal sequence of human Akt protein.
C-Terminus
Recognizes endogenous levels of total Akt protein. Does not cross-react with other related proteins. Species cross-reactivity: Mouse, rat and monkey.
Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Flow Cytometry: 1:50. Western Blot: 1:1000. Immunoprecipitation: 1:50. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

136 mM sodium chloride, 2.6 mM KCl, 12 mM sodium phosphate dibasic, pH 7.4, 2 mg/ml BSA, 50% glycerol
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months.
For research use only.

About AKT1 + AKT2 + AKT3

AKT, also known as protein kinase B (PKB), constitutes a family of serine/threonine kinases. AKT has 3 isoforms: AKT1, AKT2), and AKT3). These isoforms share a high degree of homology, especially in the catalytic domains (87%-90% identical amino acids).

Requested From: 
Date Requested: 4/25/2018

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