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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AKT1 + AKT2 + AKT3 Antibody (Ala444) LS‑C176649
AKT1 + AKT2 + AKT3 antibody LS-C176649 is an unconjugated rabbit polyclonal antibody to AKT1 + AKT2 + AKT3 from human, mouse and rat. Validated for IHC and WB.
Catalog
Size
Price
LS-C176649-100
100 µl
Unavailable

Popular AKT1 + AKT2 + AKT3 Products

Anti-AKT1/2/3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2844 dilution 1:100.
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Western blot of extracts from HeLa cells treated with heat shock, using Akt (Phospho-Ser473) Antibody. The lane on the left is treated with the synthesized peptide.
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Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine, Dog, Chicken, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemical analysis of AKT staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. w
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Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
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Species: Human, Mouse
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Product Description

AKT1 + AKT2 + AKT3 antibody LS-C176649 is an unconjugated rabbit polyclonal antibody to AKT1 + AKT2 + AKT3 from human, mouse and rat. Validated for IHC and WB.
About AKT1 + AKT2 + AKT3
AKT, also known as protein kinase B (PKB), constitutes a family of serine/threonine kinases. AKT has 3 isoforms: AKT1, AKT2), and AKT3). These isoforms share a high degree of homology, especially in the catalytic domains (87%-90% identical amino acids).

Specifications

Target
Human AKT1 + AKT2 + AKT3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide around Ala444 of human AKT
Epitope
Ala444
Specificity
Human AKT
Presentation
PBS, pH 7.2, 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of Akt (A444) pAb in paraffin-embedded human lung carcinoma tissue.
IHC of Akt (A444) pAb in paraffin-embedded human lung carcinoma tissue.

Western blot

Western blot of Akt (A444) pAb in extracts from NIH/3T3 cells.
Western blot of Akt (A444) pAb in extracts from NIH/3T3 cells.

Immunohistochemistry

IHC of Akt (A444) pAb in paraffin-embedded human lung carcinoma tissue.
IHC of Akt (A444) pAb in paraffin-embedded human lung carcinoma tissue.

Western blot

Western blot of Akt (A444) pAb in extracts from NIH/3T3 cells.
Western blot of Akt (A444) pAb in extracts from NIH/3T3 cells.

Immunohistochemistry

IHC of Akt (A444) pAb in paraffin-embedded human lung carcinoma tissue.
IHC of Akt (A444) pAb in paraffin-embedded human lung carcinoma tissue.

Western blot

Western blot of Akt (A444) pAb in extracts from NIH/3T3 cells.
Western blot of Akt (A444) pAb in extracts from NIH/3T3 cells.

Immunohistochemistry

IHC of Akt (A444) pAb in paraffin-embedded human lung carcinoma tissue.
IHC of Akt (A444) pAb in paraffin-embedded human lung carcinoma tissue.

Western blot

Western blot of Akt (A444) pAb in extracts from NIH/3T3 cells.
Western blot of Akt (A444) pAb in extracts from NIH/3T3 cells.


Requested From: 
Date Requested: 6/20/2018

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