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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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AKAP8 / AKAP95 Antibody (aa576‑625) LS‑C109593
AKAP95 antibody LS-C109593 is an unconjugated rabbit polyclonal antibody to AKAP95 (AKAP8) from human, dog, horse and other species. Validated for WB.
Catalog
Size
Price
LS-C109593-50
50 µg
Unavailable

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Product Description

AKAP95 antibody LS-C109593 is an unconjugated rabbit polyclonal antibody to AKAP95 (AKAP8) from human, dog, horse and other species. Validated for WB.
About AKAP8 / AKAP95
AKAP8 / AKAP95 is a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. O43823 NM_005858 NP_005849.1

AKAP8 Antibody, A-kinase anchor protein 8 Antibody, A-kinase anchor protein, 95kDa Antibody, A-kinase anchor protein 95 kDa Antibody, AKAP-8 Antibody, AKAP95 Antibody, AKAP 95 Antibody, AKAP-95 Antibody


Specifications

Target
Human AKAP8 / AKAP95
Host
Rabbit
Reactivity
Human, Dog, Horse, Pig (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bovine (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
AKAP8 / AKAP95 antibody was raised against synthetic peptide located between aa576-625 of human AKAP8 (O43823, NP_005849). Percent identity by BLAST analysis: Human, Gibbon, Marmoset, Panda, Dog, Horse (100%); Gorilla, Monkey, Bovine (92%); Elephant (86%); Pig (85%).
Blocking Peptide
LS-E11002 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C109593. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa576-625
Specificity
Human AKAP8
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

AKAP8 antibody LS-C109593 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
AKAP8 antibody LS-C109593 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

AKAP8 antibody LS-C109593 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
AKAP8 antibody LS-C109593 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

AKAP8 antibody LS-C109593 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
AKAP8 antibody LS-C109593 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

AKAP8 antibody LS-C109593 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
AKAP8 antibody LS-C109593 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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