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Anti-AKAP8 / AKAP95 Antibody (aa576-625) LS-C109593

Catalog	Size	Price
LS-C109593-50	50 µg	Unavailable

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Western blot of Jurkat cell lysate using AKAP8 Antibody

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Format:	Unconjugated

AKAP8 antibody LS-C109593 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Details

Target:	Human AKAP8 / AKAP95
Host:	Rabbit
Reactivity:	Human, Dog, Horse, Pig (tested or 100% immunogen sequence identity)
Predicted:	Monkey, Bovine (at least 90% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.2 - 1 µg/ml)
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

AKAP8 / AKAP95 antibody was raised against synthetic peptide located between aa576-625 of human AKAP8 (O43823, NP_005849). Percent identity by BLAST analysis: Human, Gibbon, Marmoset, Panda, Dog, Horse (100%); Gorilla, Monkey, Bovine (92%); Elephant (86%); Pig (85%).

Blocking Peptide:

LS-E11002 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C109593. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

aa576-625

Specificity:

Human AKAP8

Usage:

Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About AKAP8 / AKAP95

Uniprot: O43823 NCBI: NM_005858 NP_005849.1

Synonyms: AKAP8 Antibody, A-kinase anchor protein 8 Antibody, A-kinase anchor protein, 95kDa Antibody, A-kinase anchor protein 95 kDa Antibody, AKAP-8 Antibody, AKAP95 Antibody, AKAP 95 Antibody, AKAP-95 Antibody

Description: AKAP8 / AKAP95 is a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin.

(More About AKAP8 / AKAP95)

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Western blot

AKAP8 antibody LS-C109593 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 4/26/2018

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