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Anti-AIM2 Antibody (aa4-32) LS-C156196

Ordering

Wt. Vol. Conc. Price
- 400 µl - Unavailable

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Anti-AIM2 Antibody (aa4-32) LS-C156196
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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human AIM2
Human
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human AIM2
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

LS-E10367 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C156196. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa4-32
This AIM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human AIM2.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About AIM2

O14862 NM_004833 NP_004824.1

AIM2 Antibody, Absent in melanoma 2 Antibody, PYHIN4 Antibody

Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of AIM2 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot
Western blot of AIM2 Antibody in MCF-7, HL-60, K562, HeLa cell line lysates (35 ug/lane). AIM2 (arrow) was detected using the purified antibody.

Flow Cytometry

Flow Cytometry
AIM2 Antibody flow cytometry of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: 
Date Requested: 4/23/2017

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