Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal to Yeast ADH / Alcohol Dehydrogenase
Western blot, Immunoprecipitation, ELISA
Yeast ADH / Alcohol Dehydrogenase
Yeast (tested or 100% immunogen sequence identity)
Delipidated and defibrinated
Western blot (1:500 - 1:2000)
ELISA (1:5000 - 1:25000)
Specificity and Use
Full length Alcohol Dehydrogenase isolated from yeast.
Alcohol Dehydrogenase [Yeast]. Cross reactivity against Alcohol Dehydrogenase from most fungal sources is likely due to sequence homology as determined by BLAST analysis. Cross reactivity with Alcohol Dehydrogenase from other sources is unknown
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
Anti-Alcohol Dehydrogenase Antibody - Western Blot. Western blot analysis with Anti-Alcohol Dehydrogenase antibody was used to detect yeast Alcohol Dehydrogenase. Comparison to molecular weight markers (not shown) indicates estimated molecular weights consistent with monomer, dimer and tetramer present in this preparation. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min at room temperature. The IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.