Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat, Mouse (tested or 100% immunogen sequence identity)
Human (at least 90% immunogen sequence identity)
Western blot (1 - 10 µg/ml)
Specificity and Use
ADAM17 / TACE antibody was raised against a 17 aa peptide near the C-terminus of rat TACE (1) conjugated to KLH.
The rat peptide sequence is 100% conserved in mouse and 99% in human TACE. No significant sequence homology exists with other proteases. Antibody cross-reactivity in various species is not established.
Suitable for use in ELISA and Western Blot. Western Blot: 1-10 ug/ml using ECL.
Tumor-necrosis factor-a is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-a is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-a. TNF-a converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately.