Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal (IgG) to Human ACHE / Acetylcholinesterase
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Mouse Monoclonal [clone AE-2] (IgG1) to Human ACHE / Acetylcholinesterase
Human, Monkey, Rat
IHC, Western blot, Immunoprecipitation
Human ACHE / Acetylcholinesterase
Human, Monkey, Rat (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [AE-2]
IHC (1:200 - 1:1000)
Western blot (1:500 - 1:1000)
Specificity and Use
Acetylcholinesterase AChE from human erythrocyte
Acetylcholinesterase from human and monkey
Immunohistochemistry (unfixed):1:200-1:1000 does not react with fixed tissue; add antibody to perfused but unfixed tissue, then fix after primary incubation. Western blot: 1:500-1:1000 on SKBR-3 cells. Immunoprecipitation: use 0.5% triton X-100 extracts a
0.02 M PBS, 0.25 M sodium chloride, pH 7.2, 0.1% sodium azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles. Store undiluted.
Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface.