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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Acetylcholinesterase (AChE) Assay Kit (Colorimetric) - LS-K222

Available for shipment within the USA only
Catalog
Size
Price
LS-K222-100
100 tst
$385

Available for shipment within the USA only

Product Description

ACETYLCHOLINESTERASE (EC 3.1.1.7, AChE), also known as RBC cholinesterase, is found primarily in the blood and neural synapses. Low serum cholinesterase activity may relate to exposure to insecticides or to one of a number of variant genotypes. AChE catalyzes the hydrolysis of the neurotransmitter acetylcholine into choline and acetic acid, a reaction necessary to allow a cholinergic neuron to return to its resting state after activation. Cholinesterase levels of cells and plasma are used as a guide in establishing safety precautions relative to exposure and contact, as well as a guide in determining the need for workers to be removed from areas of contact with the organic phosphate insecticides. Simple, direct and automation-ready procedures for measuring AChE activity are very desirable. This Acetylcholinesterase Assay is based on an improved Ellman Method,in which thiocholine produced by the action of acetylcholinesterase forms a yellow color with 5,5'-dithiobis(2-nitrobenzoic acid). The intensity of the product color, measured at 412 nm, is proportionate to the enzyme activity in the sample.

Specifications

Name
Acetylcholinesterase (AChE) Assay Kit (Colorimetric)
Product Type
Detection/Quantition
Usage Summary
For quantitative determination of acetylcholinesterase activity and evaluation of acetylcholinesterase inhibitors.
Target
ACHE / Acetylcholinesterase
Sample Type
Blood, Plasma, Serum
Detection
Colorimetric (412 nm Absorbance)
Kit Components
  • Assay Buffer (pH 7.5)
  • Reagent
  • ibrator (equivalent to 200 U/L)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped Ambient, Store at Store at -20°C., 6 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.
    About ACHE / Acetylcholinesterase
    P22303 NM_015831 NP_056646.1

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    Requested From: United States
    Date Requested: 3/23/2019
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