ASIC3 antibody LS-C11723 is an unconjugated guinea pig polyclonal antibody to rat ASIC3 (ACCN3). Validated for ICC and IHC.
(tested or 100% immunogen sequence identity)
Mouse (at least 90% immunogen sequence identity)
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ACCN3 / ASIC3 antibody was raised against synthetic peptide (C-TASLDPDDFDPEPSDPLGSP) residues 285-304 from the extracellular domain of rat ASIC3. Percent identity by BLAST analysis: Rat (100%); Mouse (90%).
Recognizes rat ASIC3.
Suitable for use in Immunohistochemistry and Immunocytochemistry. Immunohistochemistry (perfusion fixed tissue): 1:50. Perfusion: 1) calcium-free Tyrode's solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose, PBS. Immunocytochemistry: 1:50. ASIC3 transfected cells and primary neuronal cell cultures.
PBS, pH 7.4, 0.05% Sodium Azide, 40% Glycerol, 1% BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.