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Catalog Number Size Price
LS-C744901-25 25 µl (1 mg/ml) $275 
AKT3 Antibody - Immunofluorescence of Mouse monoclonal anti-AKT3 antibody Cell Type: A431 cells Fixation: 4% paraformaldehyde 10 min Permeablization: 0.5% Triton X 30 min Primary Ab: 200-301-J36 1:250 72 hours 4°C Secondary Ab: 610-142-121 1:1000 Overnight 4°C
AKT3 Antibody - Western Blot of Mouse monoclonal anti-AKT3 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Load: 50 ng per lane. Primary antibody: anti-AKT3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Rabbit secondary antibody anti mouse at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 85 kDa for GST-AKT3.
AKT3 Antibody - ELISA of Mouse Monoclonal anti-AKT3 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Mouse monoclonal anti-AKT3 antibody at 100ng/well. Dilution series: 2-fold. Mid-point concentration: 3 ng/mL Mouse monoclonal anti-AKT3 antibody. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB
AKT3 Antibody - Immunofluorescence of Mouse monoclonal anti-AKT3 antibody Cell Type: A431 cells Fixation: 4% paraformaldehyde 10 min Permeablization: 0.5% Triton X 30 min Primary Ab: 200-301-J36 1:250 72 hours 4°C Secondary Ab: 610-142-121 1:1000 Overnight 4°C
AKT3 Antibody - Western Blot of Mouse monoclonal anti-AKT3 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Load: 50 ng per lane. Primary antibody: anti-AKT3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Rabbit secondary antibody anti mouse at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 85 kDa for GST-AKT3.
AKT3 Antibody - ELISA of Mouse Monoclonal anti-AKT3 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Mouse monoclonal anti-AKT3 antibody at 100ng/well. Dilution series: 2-fold. Mid-point concentration: 3 ng/mL Mouse monoclonal anti-AKT3 antibody. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB
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Monoclonal Mouse anti‑Human AKT3 Antibody (clone 9A5.H9.G7, Internal, IHC, IF, WB) LS‑C744901

Monoclonal Mouse anti‑Human AKT3 Antibody (clone 9A5.H9.G7, Internal, IHC, IF, WB) LS‑C744901

Antibody:
AKT3 Mouse anti-Human Monoclonal (Internal) (9A5.H9.G7) Antibody
Application:
IHC, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C744901-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
AKT3 Mouse anti-Human Monoclonal (Internal) (9A5.H9.G7) Antibody
Application:
IHC, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
AKT3 antibody LS-C744901 is an unconjugated mouse monoclonal antibody to human AKT3 (Internal). Validated for ELISA, Flow, IF, IHC and WB.
Target
Human AKT3
Synonyms
AKT3 | AKT3/PKBgamma | PRKBG | PKB gamma | Protein kinase Akt-3 | RAC-gamma | MPPH | PKB-GAMMA | PKBG | Protein kinase B gamma | RAC-PK-gamma | STK-2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
9A5.H9.G7
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Anti-AKT3 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.
Epitope
Internal
Specificity
The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.
Applications
  • IHC (20 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry
  • ELISA (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT3
Q9Y243 NM_005465 NP_005456.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/17/2021