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Catalog Number Size Price
LS-B1319-50 50 µg (0.5 mg/ml) $440 
AKT3 Antibody - Anti-AKT3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
AKT3 Antibody - Western blot analysis for AKT3 using antibody at 2 ug/ml dilution against 30 ug/lane of human kidney lysate .
AKT3 Antibody - Anti-AKT3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
AKT3 Antibody - Western blot analysis for AKT3 using antibody at 2 ug/ml dilution against 30 ug/lane of human kidney lysate .
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IHC‑plus™ Monoclonal Mouse anti‑Human AKT3 Antibody (clone 66C1247.1, aa119‑136, IHC, WB) LS‑B1319

IHC‑plus™ Monoclonal Mouse anti‑Human AKT3 Antibody (clone 66C1247.1, aa119‑136, IHC, WB) LS‑B1319

Note: This antibody replaces LS-C497
Antibody:
AKT3 Mouse anti-Human Monoclonal (aa119-136) (66C1247.1) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-B1319-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
AKT3 Mouse anti-Human Monoclonal (aa119-136) (66C1247.1) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
AKT3 antibody LS-B1319 is an unconjugated mouse monoclonal antibody to AKT3 (aa119-136) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human AKT3
Synonyms
AKT3 | AKT3/PKBgamma | PRKBG | PKB gamma | Protein kinase Akt-3 | RAC-gamma | MPPH | PKB-GAMMA | PKBG | Protein kinase B gamma | RAC-PK-gamma | STK-2
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Predicted
Hamster (at least 90% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
66C1247.1
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to amino acid residues 119-136 (CSPTSQIDNIGEEEMDAS) of human AKT3, GenBank no. gi|4574744|gb|AAD24196.1|AF135794_1. This sequence is identical in human, mouse, rat, cow, dog, and chicken. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%); Hamster (94%); Zebrafish (89%).
Epitope
aa119-136
Specificity
A synthetic peptide corresponding to amino acid residues 119-136 (CSPTSQIDNIGEEEMDAS) of human Akt3, GenBank no. gi|4574744|gb|AAD24196.1|AF135794_1. This sequence is identical in human, mouse, rat, cow, dog, and chicken.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B1319 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1319 was determined to be 10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 0.05% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT3
Q9Y243 NM_005465 NP_005456.1

Validation

AKT3 Antibody - Anti-AKT3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-AKT3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-AKT3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ AKT3 Antibody (aa119-136, clone 66C1247.1) for IHC, WB/Western LS-B1319 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/19/2021