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Catalog Number Size Price
LS-C745288-25 25 µl (1 mg/ml) $304 
AKT1 Antibody - Immunohistochemistry of Rabbit Anti-AKT pT308 Antibody. Tissue: human breast tissue (lymph nodes). Antigen retrieval: HIER using citrate buffer for 20 minutes. Fixative: None. Primary Antibody: Anti-AKT phosphoT308 at 1:200 for 30 minutes at RT. Secondary Antibody: Anti-Rabbit Poly-HRP-IgG Ready to Use for 8 minutes at RT. Counterstain: Hematoxylin. Substrate: DAB. Analysis: Strong staining in nucleus. May be suitable with more dilutions.
AKT1 Antibody - Western Blot of rabbit AKT Antibody. Lane 1: NIR MW protein ladder. Lane 2: AKT1, recombinant. Lane 3: AKT1, phosphatase-treated. Lane 4: AKT1, mutant T308A/S473A. Lane 5: AKT2, recombinant. Lane 6: AKT2, phosphatase-treated. Lane 7: AKT3, recombinant. Lane 8: AKT3, phosphatase-treated. Load: 50ng per lane. Blot A: 600-401-269 Anti-Akt pT308 used at 1:2270, Blot B: 100-401-401 Anti-Akt used 1:1000.
AKT1 Antibody - Western Blot of rabbit anti-Akt phospho T308 antibody. Lane 1: GST tagged AKT1 un-active recombinant protein. Lane 2: GST tagged AKT1 active recombinant protein. Load: 50 ng per lane. Primary antibody: Akt phospho T308 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Other band(s): none.
AKT1 Antibody - Immunohistochemistry of Rabbit Anti-AKT pT308 Antibody. Tissue: human breast tissue (lymph nodes). Antigen retrieval: HIER using citrate buffer for 20 minutes. Fixative: None. Primary Antibody: Anti-AKT phosphoT308 at 1:200 for 30 minutes at RT. Secondary Antibody: Anti-Rabbit Poly-HRP-IgG Ready to Use for 8 minutes at RT. Counterstain: Hematoxylin. Substrate: DAB. Analysis: Strong staining in nucleus. May be suitable with more dilutions.
AKT1 Antibody - Western Blot of rabbit AKT Antibody. Lane 1: NIR MW protein ladder. Lane 2: AKT1, recombinant. Lane 3: AKT1, phosphatase-treated. Lane 4: AKT1, mutant T308A/S473A. Lane 5: AKT2, recombinant. Lane 6: AKT2, phosphatase-treated. Lane 7: AKT3, recombinant. Lane 8: AKT3, phosphatase-treated. Load: 50ng per lane. Blot A: 600-401-269 Anti-Akt pT308 used at 1:2270, Blot B: 100-401-401 Anti-Akt used 1:1000.
AKT1 Antibody - Western Blot of rabbit anti-Akt phospho T308 antibody. Lane 1: GST tagged AKT1 un-active recombinant protein. Lane 2: GST tagged AKT1 active recombinant protein. Load: 50 ng per lane. Primary antibody: Akt phospho T308 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Other band(s): none.
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Polyclonal Rabbit anti‑Human AKT1 Antibody (phospho‑Thr308, IHC, WB) LS‑C745288

Polyclonal Rabbit anti‑Human AKT1 Antibody (phospho‑Thr308, IHC, WB) LS‑C745288

Antibody:
AKT1 Rabbit anti-Human Polyclonal (pThr308) Antibody
Application:
IHC, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$304
LS-C745288-25
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
AKT1 Rabbit anti-Human Polyclonal (pThr308) Antibody
Application:
IHC, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
AKT1 antibody LS-C745288 is an unconjugated rabbit polyclonal antibody to AKT1 (pThr308) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IHC and WB.
Target
Human AKT1
Synonyms
AKT1 | AKT | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Anti-AKT pT308 polyclonal antibody was produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to residues surrounding threonine 308 of human AKT1 protein.
Epitope
pThr308
Specificity
Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated human AKT. Minimal reactivity occurs against non-phosphorylated AKT. Reactivity against AKT from other species may occur but has not yet been tested.
Applications
  • IHC (1:200)
  • Western blot (1:1000)
  • Flow Cytometry
  • ELISA (1:15000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT1
P31749 NM_005163 NP_005154.2

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Requested From: United States
Date Requested: 5/2/2024