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Catalog Number Size Price
LS-C744916-50 50 µg (1 mg/ml) $492 
AKT1 Antibody - Western Blot of Mouse anti-AKT1 antibody. Lane 1: AKT1 Null. Lane 2: WT. Lane 3: MEF #1. Lane 4 : A549. Lane 5: Calu-1. Lane 6: PC-3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Lane 11: C2C12. Load: 20 ug per lane. Primary antibody: AKT1 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 56 kDa for AKT1. Other band(s): none.
AKT1 Antibody - Western Blot of Mouse anti-AKT1 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT1 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT2. Other band(s): none.
AKT1 Antibody - Flow Cytometry of Mouse anti-AKT1 antibody. Cells: LNCap Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT1 antibody at 1.0 µg/mL for 20 min at 4°C.
AKT1 Antibody - Plate was coated with monoclonal anti AKT1 antibody followed by incubation with recombinant AKT1, AKT2, AKT3 proteins. Binding was detected with biotinylated monoclonal anti AKT pS473. The signal shows specificity of the monoclonal anti-AKT1 antibody to recombinant isoform AKT1 protein and not the isoform 2 and 3
AKT1 Antibody - Western Blot of Mouse anti-AKT1 antibody. Lane 1: AKT1 Null. Lane 2: WT. Lane 3: MEF #1. Lane 4 : A549. Lane 5: Calu-1. Lane 6: PC-3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Lane 11: C2C12. Load: 20 ug per lane. Primary antibody: AKT1 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 56 kDa for AKT1. Other band(s): none.
AKT1 Antibody - Western Blot of Mouse anti-AKT1 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT1 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT2. Other band(s): none.
AKT1 Antibody - Flow Cytometry of Mouse anti-AKT1 antibody. Cells: LNCap Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT1 antibody at 1.0 µg/mL for 20 min at 4°C.
AKT1 Antibody - Plate was coated with monoclonal anti AKT1 antibody followed by incubation with recombinant AKT1, AKT2, AKT3 proteins. Binding was detected with biotinylated monoclonal anti AKT pS473. The signal shows specificity of the monoclonal anti-AKT1 antibody to recombinant isoform AKT1 protein and not the isoform 2 and 3
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Monoclonal Mouse anti‑Human AKT1 Antibody (clone 14E5.A2.B2.H9, FITC, Internal, IHC, IF, WB) LS‑C744916

Monoclonal Mouse anti‑Human AKT1 Antibody (clone 14E5.A2.B2.H9, FITC, Internal, IHC, IF, WB) LS‑C744916

Antibody:
AKT1 Mouse anti-Human Monoclonal (Internal) (FITC) (14E5.A2.B2.H9) Antibody
Application:
IHC, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
FITC, Unmodified
Price
Catalog Number
$492
LS-C744916-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
AKT1 Mouse anti-Human Monoclonal (Internal) (FITC) (14E5.A2.B2.H9) Antibody
Application:
IHC, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
FITC, Unmodified

Specifications

Description
AKT1 antibody LS-C744916 is an FITC-conjugated mouse monoclonal antibody to AKT1 (Internal) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IF, IHC and WB.
Target
Human AKT1
Synonyms
AKT1 | AKT | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
14E5.A2.B2.H9
Conjugations
FITC
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein followed by monoclonal development.
Epitope
Internal
Specificity
The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.
Applications
  • IHC
  • Immunofluorescence
  • Western blot
  • Flow Cytometry
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 50µL of deionized water.
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT1
P31749 NM_005163 NP_005154.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021