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Catalog Number Size Price
LS-C745007-25 25 µl (1 mg/ml) $275 
AKT1 Antibody - Western Blot of Anti-AKT (RABBIT) Antibody. Lane 1: SuperSignal MW markers. Lane 2: rAKT1. Lane 3: rAKT2. Lane 4: rAKT3. Load: 50 ng per lane. Primary antibody: Anti- AKT (RABBIT) Antibody at 1:1,000 for 2 hours at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:20,000 for 1 hour at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070), 1/2 hour at RT. Predicted/Observed size: 56kDa, 56kDa for AKT1, 2, and 3.

Polyclonal Rabbit anti‑Human AKT1 Antibody (IHC, WB) LS‑C745007

Polyclonal Rabbit anti‑Human AKT1 Antibody (IHC, WB) LS‑C745007

Antibody:
AKT1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745007-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
AKT1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
AKT1 antibody LS-C745007 is an unconjugated rabbit polyclonal antibody to AKT1 from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IHC and WB.
Target
Human AKT1
Synonyms
AKT1 | AKT | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Anti-AKT Pan Reactive Antibody was produced in rabbits by repeated immunizations with a cocktail containing recombinant human AKT1, AKT2, and AKT3 protein isoforms.
Specificity
Reacts with the AKT from human tissues. Anti-AKT antibody was purified from monospecific antiserum by Protein A chromatography. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.
Applications
  • IHC
  • Western blot (1:1000 - 1:5000)
  • Flow Cytometry
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT1
P31749 NM_005163 NP_005154.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021