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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C18770-500 500 µg (5 mg/ml) $475 
ADGRE5 / CD97 Antibody - Anti-CD97 Antibody - Western Blot. Western blot of Protein A purified anti-CD97 antibody shows detection of bands corresponding to free Fc-CD97- (5EGF) (lower arrowhead) and Fc-CD97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells. The left lane contains lysate from cells transfected with control DNA. The right lane contains lysate from COS cells expressing Fc-CD97- (5EGF). No staining was noted from bone marrow lysates taken from CD97 knockout mice. The identity of the band at ~65 kD appearing in all lanes is not known. The formation of the CD97 complex is currently under investigation. Approximately 10 ul of lysate was used in each lane. A 1:1000 dilution of the primary antibody was used. The image was processed using a 10-sec exposure. Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.

Polyclonal ADGRE5 / CD97 Antibody (IF, WB) LS‑C18770

Polyclonal ADGRE5 / CD97 Antibody (IF, WB) LS‑C18770

Antibody:
Rabbit Polyclonal to Mouse ADGRE5 / CD97
Application:
IF, WB, ELISA
Reactivity:
Mouse, Human, Chimpanzee
Format:
Unconjugated, Unmodified
Price
Catalog Number
$475 
LS-C18770-500
500 µg
5 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Mouse ADGRE5 / CD97
Application:
IF, WB, ELISA
Reactivity:
Mouse, Human, Chimpanzee
Format:
Unconjugated, Unmodified

Specifications

Description
CD97 antibody LS-C18770 is an unconjugated rabbit polyclonal antibody to CD97 (ADGRE5) from human, mouse and chimpanzee. Validated for ELISA, IF and WB.
Target
Mouse ADGRE5 / CD97
Synonyms
ADGRE5 | CD97 | CD97 molecule | Leukocyte antigen CD97 | TM7LN1 | CD97 antigen
Host
Rabbit
Reactivity
Mouse, Human, Chimpanzee (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:1000 - 1:5000)
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:10000 - 1:50000)
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-512 (extracellular domain) of mouse CD97 protein.
Usage
This Protein A purified antibody has been tested for use in immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 81 kD in size corresponding to isoform 1 of CD97 protein by western blotting in the appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at 4°C prior to opening. Restore with 0.1 ml of deionized water or equivalent. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADGRE5 / CD97
P48960 NM_078481 NP_510966.1

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Requested From: United States
Date Requested: 3/29/2020