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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B1780-50 50 µg (0.5 mg/ml) $425 
ADGRE5 / CD97 Antibody - Anti-CD97 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
ADGRE5 / CD97 Antibody - Western blot of human Leukocyte Antigen CD97 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at 2 ug/ml.
ADGRE5 / CD97 Antibody - Anti-CD97 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
ADGRE5 / CD97 Antibody - Western blot of human Leukocyte Antigen CD97 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at 2 ug/ml.
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IHCPlus™ Polyclonal Rabbit anti‑Human ADGRE5 / CD97 Antibody (aa220‑270, IHC, WB) LS‑B1780

IHCPlus™ Polyclonal Rabbit anti‑Human ADGRE5 / CD97 Antibody (aa220‑270, IHC, WB) LS‑B1780

Note: This antibody replaces LS-C47291
Antibody:
Rabbit Polyclonal to Human ADGRE5 / CD97
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B1780-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human ADGRE5 / CD97
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
CD97 antibody LS-B1780 is an unconjugated rabbit polyclonal antibody to CD97 (ADGRE5) (aa220-270) from human. It is reactive with human and monkey. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ADGRE5 / CD97
Synonyms
ADGRE5 | CD97 | CD97 molecule | Leukocyte antigen CD97 | TM7LN1 | CD97 antigen
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 3 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A portion of amino acids 220-270of human Leukocyte Antigen CD97.
Epitope
aa220-270
Specificity
A portion of amino acids 220-270 of human Leukocyte Antigen CD97.
Usage
Immunohistochemistry: LS-B1780 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1780 was determined to be 10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADGRE5 / CD97
P48960 NM_078481 NP_510966.1

Validation

ADGRE5 / CD97 Antibody - Anti-CD97 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CD97 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CD97 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ ADGRE5 / CD97 Antibody (aa220-270) for IHC, WB/Western LS-B1780 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 7/11/2020