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IHCPlus™ ADGRE5 / CD97 Antibody aa220‑270 Polyclonal IHC,WB LS‑B1780

Catalog Number Size Price
LS-B1780-50 50 µg (0.5 mg/ml) $425 
Anti-CD97 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Western blot of human Leukocyte Antigen CD97 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at 2 ug/ml.
Anti-CD97 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Western blot of human Leukocyte Antigen CD97 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at 2 ug/ml.
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IHCPlus™ ADGRE5 / CD97 Antibody aa220‑270 Polyclonal IHC,WB LS‑B1780

Note: This antibody replaces LS-C47291
Antibody:
Rabbit Polyclonal to Human ADGRE5 / CD97
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B1780-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human ADGRE5 / CD97
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
CD97 antibody LS-B1780 is an unconjugated rabbit polyclonal antibody to CD97 (ADGRE5 ) from human and monkey. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ADGRE5 / CD97
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 3 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A portion of amino acids 220-270of human Leukocyte Antigen CD97.
Epitope
aa220-270
Specificity
A portion of amino acids 220-270 of human Leukocyte Antigen CD97.
Usage
Immunohistochemistry: LS-B1780 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1780 was determined to be 10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADGRE5 / CD97
P48960 NM_078481 NP_510966.1

Validation

Anti-CD97 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC Validated
Anti-CD97 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CD97 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

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Featured Products

Anti-CD97 Antibody - Western Blot. Western blot of Protein A purified anti-CD97 antibody shows detection of bands corresponding to free Fc-CD97- (5EGF) (lower arrowhead) and Fc-CD97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells. The left lane contains lysate from cells transfected with control DNA. The right lane contains lysate from COS cells expressing Fc-CD97- (5EGF). No staining was noted from bone marrow lysates taken from CD97 knockout mice. The identity of the band at ~65 kD appearing in all lanes is not known. The formation of the CD97 complex is currently under investigation. Approximately 10 ul of lysate was used in each lane. A 1:1000 dilution of the primary antibody was used. The image was processed using a 10-sec exposure. Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.
Species: Mouse, Human
Applications: Immunofluorescence, Western blot
Reactivity: Pig
Range: 0.156-10 ng/ml
Immunoperoxidase of monoclonal antibody to IRF5 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1.5 ug/ml]
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Reactivity: Mouse
Range: 1.6-1000 pg/ml
Reactivity: Mouse
Range: 0.27-200 pg/ml
Anti-KLKB1 antibody IHC of human small intestine, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA, Immunoelectrophoresis

Requested From: United States
Date Requested: 9/17/2019