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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ACAT2 Antibody (clone 1B7) LS-C115257

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ACAT2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-ACAT2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human prostate tissue using anti-ACAT2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human liver tissue using anti-ACAT2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human pancreas tissue using anti-ACAT2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human Kidney tissue using anti-ACAT2 mouse monoclonal antibody. (Dilution 1:50).
Anti-ACAT2 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2.
Flow cytometry of Jurkat cells, using anti-ACAT2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACAT2 antibody, and then analyzed by flow cytometry.
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ACAT2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-ACAT2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human prostate tissue using anti-ACAT2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human liver tissue using anti-ACAT2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human pancreas tissue using anti-ACAT2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human Kidney tissue using anti-ACAT2 mouse monoclonal antibody. (Dilution 1:50).
Anti-ACAT2 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2.
Flow cytometry of Jurkat cells, using anti-ACAT2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACAT2 antibody, and then analyzed by flow cytometry.
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ACAT2 Antibody (clone 1B7) LS-C115257

Antibody:
Mouse Monoclonal to Human ACAT2
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Monkey, Rat, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$345 
LS-C115257-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human ACAT2
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Monkey, Rat, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
ACAT2 antibody LS-C115257 is an unconjugated mouse monoclonal antibody to ACAT2 from human, rat, dog and other species. Validated for Flow, IF, IHC and WB.

Target
Human ACAT2
Host
Mouse
Reactivity
Human, Monkey, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
1B7
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:1000 - 1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ACAT2 antibody was raised against full length human recombinant protein of human ACAT2 (NP_0058826) produced in HEK293T cell.
Specificity
Human ACAT2
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACAT2
Q9BWD1 NM_005891 NP_005882.2

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Requested From: United States
Date Requested: 7/15/2019
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