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VPS35

Vacuolar protein sorting-associated protein 35

VPS35 belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Gene Name: Vacuolar protein sorting-associated protein 35
Synonyms: VPS35, MEM3, Maternal-embryonic 3, Vesicle protein sorting 35, PARK17, HVPS35
Target Sequences: NM_018206 NP_060676.2 Q96QK1

Publications (2)

1
Rab GTPase-activating proteins in autophagy: regulation of endocytic and autophagy pathways by direct binding to human ATG8 modifiers. Popovic D, Akutsu M, Novak I, Harper JW, Behrends C, Dikic I. Molecular and cellular biology. 2012 32:1733-44. (WB; Human) [Full Text Article] [PubMed:22354992] [PMC:PMC3347240] Related Antibodies: LS-B5909.
2
Novel Insights into the Cellular Localization and Regulation of the Autophagosomal Proteins LC3A, LC3B and LC3C. Marius W Baeken, Katja Weckmann, Philip Diefenthäler, Jan Schulte, Kamran Yusifli, Bernd Moosmann, Christian Behl, Parvana Hajieva. Cells. 2020 October;9: [Full Text Article] [PubMed:33081014] [PMC:PMC7603224] Related Antibodies: LS-B5909.

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Proteins (5)
Over-Expression Lysate (2)
Recombinant (3)
VPS35 (5)
No (5)
10His, N-terminus + Myc, C-terminus (1)
GST (1)
GST, N-terminus (1)
Myc-DDK (Flag) (2)
Human (4)
293T Cells (1)
E. coli (1)
HEK 293 Cells (1)
Wheat Germ Extract (2)
Purified (2)
VPS35 Protein - Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of Recombinant Human Vacuolar protein sorting-associated protein 35(VPS35) could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) VPS35.
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E. coli
10His, N-terminus + Myc, C-terminus
96.7 kDa
1 mg/$1,679; 100 µg/$500; 20 µg/$364
VPS35 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
91.5 kDa
20 µg/$150
VPS35 Protein - 12.5% SDS-PAGE of human VPS35 stained with Coomassie Blue
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Wheat Germ Extract
GST
118.1 kDa
10 µg/$479; 25 µg/$670
VPS35 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
VPS35 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
91.5 kDa
100 µg/$494
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).