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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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VCL / Vinculin

vinculin

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Gene Name: vinculin
Family/Subfamily: Cytoskeleton
Synonyms: VCL, CMD1W, CMH15, Metavinculin, Vinculin, MVCL
Target Sequences: NM_014000 NP_054706.1 P18206

Filtered By: None
Target
VCL / Vinculin (117)
Reactivity
Human (114)
Mouse (54)
Rat (49)
Bat (5)
Bovine (8)
Chicken (30)
Chimpanzee (3)
Dog (5)
Gibbon (3)
Guinea pig (5)
Hamster (1)
Horse (8)
Monkey (6)
Pig (16)
Rabbit (18)
Sheep (2)
Xenopus (1)
Zebrafish (8)
Application
IHC (66)
IHC-Fr (8)
IHC-P (24)
WB (109)
Flo (4)
ELISA (20)
ICC (11)
IF (28)
IP (23)
Peptide-ELISA (2)
Host
rabbit (77)
mouse (40)
Isotype
Ig (2)
IgG (46)
IgG1 (25)
IgG1,k (1)
Clonality
monoclonal mc (40)
polyclonal pc (77)
Clone
[7E10] (1)
10B2533 (1)
2C6-1B5 (1)
489220 (1)
8B5 (1)
FB11 (2)
V284 (2)
VIN-54 (4)
VLN01 (2)
Format
AP Conjugated (2)
APC (4)
APC Conjugated (6)
Azide-free (3)
Biotin Conjugated (12)
Cy3 Conjugated (4)
Cy7 Conjugated (4)
FITC Conjugated (11)
HRP Conjugated (10)
Magnetic beads Conjugated (1)
PE Conjugated (6)
Sepharose Conjugated (1)
Unconjugated (60)
Epitope
aa879-1064 (10)
aa1020-1134 (9)
C-Terminus (5)
aa873-922 (4)
760-840 aa (2)
Internal (2)
aa1016-1066 (2)
pTyr100 (2)
pTyr1065 (2)
pTyr821 (2)
pTyr822 (2)
Leu817 (1)
aa 2-235 (1)
aa1-1067 (1)
aa1012-1039 (1)
aa12-39 (1)
aa173-188 (1)
aa356-607 (1)
aa750-800 (1)
aa786-835 (1)
Publications
No (117)
Showing 1-25 of 117 results

VCL / Vinculin Antibody (aa786-835) IHC-plus™ LS-B5770
Rabbit Polyclonal (IgG) to Human VCL / Vinculin
Mouse, Rat, Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-Vinculin antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-Vinculin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-Vinculin antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-Vinculin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

VCL / Vinculin Antibody (Azide-free) IHC-plus™ LS-B4764
Mouse Monoclonal (IgG1) to Human VCL / Vinculin
Human
IF IHC IHC-P IP WB
Unconjugated, Azide-free
50µl 1mg/ml  $485.00
Anti-Vinculin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-Vinculin antibody IHC of human muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-Vinculin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-Vinculin antibody IHC of human muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

VCL / Vinculin Antibody (clone VLN01) IHC-plus™ LS-B8515
Mouse Monoclonal [clone VLN01] (IgG) to Human VCL / Vinculin
Human
IF IHC IHC-P IP WB
Unconjugated
250µl  $485.00
Anti-Vinculin antibody IHC of human heart, intercalated disk. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
Anti-Vinculin antibody IHC of human heart, intercalated disk. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.

VCL / Vinculin Antibody (Internal) IHC-plus™ LS-B12145
Rabbit Polyclonal (IgG) to Human VCL / Vinculin
Mouse, Rat, Human
ELISA ICC IF IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $425.00
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

VCL / Vinculin Antibody (aa1016-1066) IHC-plus™ LS-B12965
Rabbit Polyclonal (IgG) to Human VCL / Vinculin
Chicken, Zebrafish, Pig, Horse, Rat, Bovine, Rabbit, Mouse, Human
IF IHC IHC-P IP WB
Unconjugated
50µl 1mg/ml  $425.00
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

VCL / Vinculin Antibody (C-Terminus) IHC-plus™ LS-B10133
Rabbit Polyclonal (IgG) to Human VCL / Vinculin
Mouse, Rat, Human
ELISA IF IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $425.00
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-VCL / Vinculin antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-VCL / Vinculin antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

VCL / Vinculin Antibody (Internal) LS-C358386
Rabbit Polyclonal to Human VCL / Vinculin
Chicken, Rat, Pig, Mouse, Human
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of Vinculin staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Vinculin staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of Vinculin staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Vinculin staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

VCL / Vinculin Antibody IHC-plus™ LS-B4848
Rabbit Polyclonal to Human VCL / Vinculin
Human
IHC IHC-P WB
Unconjugated
100µl 0.73mg/ml  $425.00
Anti-Vinculin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Vinculin antibody IHC of human skin, arrector pili. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Vinculin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Vinculin antibody IHC of human skin, arrector pili. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

VCL / Vinculin Antibody (aa1-1067, clone 10B2533, Azide-free) LS-C183723
Mouse Monoclonal [clone 10B2533] (IgG1) to Human VCL / Vinculin
Human
ELISA ICC WB
Unconjugated, Azide-free
100µl 0.42mg/ml  $680.00

VCL / Vinculin Antibody (aa356-607) IHC-plus™ LS-B9609
Rabbit Polyclonal to Human VCL / Vinculin
Human
ELISA IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $425.00
Human Prostate, Smooth Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot Image
Human Prostate, Smooth Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot Image

VCL / Vinculin Antibody LS-C124793
Mouse Monoclonal (IgG1) to Human VCL / Vinculin
Chicken, Rabbit, Mouse, Rat, Human
IHC IHC-Fr WB
Unconjugated
100µg  $530.00

VCL / Vinculin Antibody (phospho-Tyr1065) LS-C40219
Rabbit Polyclonal to Human VCL / Vinculin
Chicken, Mouse, Human
IP WB
Unconjugated
10blot  $635.00

VCL / Vinculin Antibody (aa 2-235) IHC-plus™ LS-B14984
Rabbit Polyclonal (IgG) to Human VCL / Vinculin
Human
ELISA IHC IHC-P WB
Unconjugated
50µg  $425.00
Human Uterus, Myometrium: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus, Myometrium: Formalin-Fixed, Paraffin-Embedded (FFPE)

VCL / Vinculin Antibody (Biotin) LS-C211451
Rabbit Polyclonal (IgG) to Human VCL / Vinculin
Human
ELISA WB
Biotin Conjugated
50µg  $285.00; 100µg 0.25mg/ml  $385.00

VCL / Vinculin Antibody (clone VIN-54) LS-C124795
Mouse Monoclonal [clone VIN-54] (IgG) to Human VCL / Vinculin
Chicken, Mouse, Rat, Human
WB
Unconjugated
100µg  $590.00

VCL / Vinculin Antibody LS-C669017
Rabbit Polyclonal (IgG) to Human VCL / Vinculin
Human
ELISA ICC IF IHC IHC-P WB
Unconjugated
0.1mg  $365.00
Immunocytochemistry of Vinculin in Jurkat cells with Vinculin antibody at 5 µg/ml.
Immunofluorescence of Vinculin in Jurkat cells with Vinculin antibody at 20 µg/ml.
Immunocytochemistry of Vinculin in Jurkat cells with Vinculin antibody at 5 µg/ml.
Immunofluorescence of Vinculin in Jurkat cells with Vinculin antibody at 20 µg/ml.

VCL / Vinculin Antibody (C-Terminus) LS-C345584
Rabbit Polyclonal to Human VCL / Vinculin
Human
WB
Unconjugated
50µg  $365.00
Western blot of VINC Antibody with human PANC1 Whole Cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of VINC Antibody with human PANC1 Whole Cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

VCL / Vinculin Antibody LS-C393258
Rabbit Polyclonal to Human VCL / Vinculin
Mouse, Rat, Human
ICC IF IHC IHC-P WB
Unconjugated
100µl  $305.00

VCL / Vinculin Antibody (phospho-Tyr821) LS-C416583
Rabbit Polyclonal to Human VCL / Vinculin
Mouse, Rat, Human
ICC IF IHC WB
Unconjugated
100µg 1mg/ml  $305.00
Western blot of Vinculin phosphorylation expression in forskolin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot of Vinculin phosphorylation expression in forskolin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

VCL / Vinculin Antibody LS-C757626
Rabbit Polyclonal (Ig) to Human VCL / Vinculin
Mouse, Rat, Human
ELISA Flo IHC-P WB
Unconjugated
50µl  $215.00; 200µl  $325.00
Vinculin Antibody staining Vinculin in mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Vinculin Antibody staining Vinculin in mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Vinculin Antibody staining Vinculin in mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Vinculin Antibody staining Vinculin in mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

VCL / Vinculin Antibody (clone VLN01, Azide-free) LS-C87611
Mouse Monoclonal [clone VLN01] (IgG) to Human VCL / Vinculin
Human
IF IHC IHC-P IP WB
Unconjugated, Azide-free
0.1ml  $615.00; 0.2ml  $1,005.00

VCL / Vinculin Antibody (760-840 aa) LS-C386654
Rabbit Polyclonal (IgG) to Human VCL / Vinculin
Mouse, Rat, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Vinculin antibody
Western blot of Vinculin antibody

VCL / Vinculin Antibody (clone V284) LS-C745676
Mouse Monoclonal [clone V284] (IgG1) to Human VCL / Vinculin
Human
Flo IF IHC-Fr IHC-P IP WB
Unconjugated
0.1mg  $305.00

VCL / Vinculin Antibody LS-C40210
Mouse Monoclonal (IgG1) to Human VCL / Vinculin
Chicken, Rabbit, Mouse, Rat, Human
ICC IHC IHC-Fr IP WB
Unconjugated
200µg  $650.00

VCL / Vinculin Antibody LS-C757625
Rabbit Polyclonal (Ig) to Human VCL / Vinculin
Mouse, Rat, Human
ELISA Flo IHC-P WB
Unconjugated
50µl  $215.00; 200µl  $325.00
Vinculin Antibody staining Vinculin in mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes: Anti-Vinculin at 1:2000 dilution Lane 1: human skeletal muscle lysate Lane 2: A431 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: mouse kidney lysate Lane 5: PC-12 whole cell lysate Lane 6: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 117 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Vinculin Antibody staining Vinculin in mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes: Anti-Vinculin at 1:2000 dilution Lane 1: human skeletal muscle lysate Lane 2: A431 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: mouse kidney lysate Lane 5: PC-12 whole cell lysate Lane 6: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 117 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Showing 1-25 of 117 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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