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UBE2O

ubiquitin-conjugating enzyme E2O

E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASH1, leading to promote endosomal F-actin assembly (PubMed:23452853).

Gene Name: ubiquitin-conjugating enzyme E2O
Synonyms: UBE2O, KIAA1734, Ubiquitin-protein ligase O, Ubiquitin carrier protein O, E2-230K
Target Sequences: NM_022066 NP_071349.3 Q9C0C9

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Antibodies (15)
Primary (15)
UBE2O (15)
Human (15)
Mouse (6)
Rat (3)
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Chicken (3)
Chimpanzee (3)
Dog (3)
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Guinea pig (3)
Horse (3)
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IHC (2)
IHC-P (1)
WB (13)
ELISA (6)
IP (1)
Peptide-ELISA (1)
rabbit (14)
goat (1)
Product Group
IHCPlus (1)
IgG (8)
polyclonal pc (15)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
Unconjugated (9)
aa1079-1128 (4)
aa1279-1292 (1)
aa725-775 (1)
No (15)
UBE2O Antibody - Anti-UBE2O antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
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UBE2O Goat anti-Human Polyclonal (aa1279-1292) Antibody
Human, Monkey
IHC, IHC-P, Peptide-ELISA
Unconjugated
50 µg/$485
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UBE2O Rabbit anti-Human Polyclonal (aa725-775) Antibody
Mouse, Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
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UBE2O Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$351
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UBE2O Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, WB
Unconjugated
100 µg/$357
UBE2O Antibody - Western blot. All lanes: UBE2O antibody at 0.8 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: A431 whole cell lysate. Secondary Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 141 kDa. Observed band size: 141 kDa.
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UBE2O Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
50 µg/$294; 100 µg/$360
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UBE2O Rabbit anti-Human Polyclonal (HRP) Antibody
Human
ELISA, WB
HRP Conjugated
50 µg/$294; 100 µg/$360
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UBE2O Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
50 µg/$294; 100 µg/$360
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UBE2O Rabbit anti-Human Polyclonal (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
50 µg/$294; 100 µg/$360
UBE2O Antibody - Immunohistochemistry of paraffin-embedded Human colon carcinoma using UBE2O Polyclonal Antibody at dilution of 1:100 (40x lens).
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UBE2O Rabbit anti-Human Polyclonal Antibody
Human
IHC
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
UBE2O Antibody - UBE2O antibody Western blot of RPMI-8226 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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UBE2O Rabbit anti-Human Polyclonal (aa1079-1128) Antibody
Human
WB
Unconjugated
100 µl/$424
UBE2O Antibody - UBE2O antibody Western blot of RPMI-8226 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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UBE2O Rabbit anti-Human Polyclonal (aa1079-1128) (HRP) Antibody
Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Pig, Horse, Bat, Rat, Chimpanzee, Gibbon, Human, Monkey
WB
HRP Conjugated
100 µl/$469
UBE2O Antibody - UBE2O antibody Western blot of RPMI-8226 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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UBE2O Rabbit anti-Human Polyclonal (aa1079-1128) (Biotin) Antibody
Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Pig, Horse, Bat, Rat, Chimpanzee, Gibbon, Human, Monkey
WB
Biotin Conjugated
100 µl/$469
UBE2O Antibody - UBE2O antibody Western blot of RPMI-8226 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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UBE2O Rabbit anti-Human Polyclonal (aa1079-1128) (FITC) Antibody
Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Pig, Horse, Bat, Rat, Chimpanzee, Gibbon, Human, Monkey
WB
FITC Conjugated
100 µl/$469
UBE2O Antibody
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UBE2O Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
50 µl/$286; 100 µl/$321
UBE2O Antibody - Western blot analysis of extracts of various cell lines, using UBE2O antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
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UBE2O Rabbit anti-Human Polyclonal Antibody
Mouse, Human
WB
Unconjugated
20 µl/$287; 100 µl/$431
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).