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UBE2D1 / UBCH5

ubiquitin-conjugating enzyme E2D 1

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene.

Gene Name: ubiquitin-conjugating enzyme E2D 1
Synonyms: UBE2D1, SFT, Ubiquitin-protein ligase D1, UBC4/5 homolog, UBCH5, UBCH5A, UBC4/5, UBC5A, Ubiquitin carrier protein D1, E2(17)KB1, Stimulator of Fe transport
Target Sequences: NM_003338 NP_003329.1 P51668

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Products
Proteins (10)
Over-Expression Lysate (2)
Recombinant (8)
UBE2D1 / UBCH5 (10)
No (10)
10His, N-terminus + Myc, C-terminus (1)
GST (1)
GST, N-terminus (3)
His (2)
Myc-DDK (Flag) (3)
Human (8)
Oryza sativa (1)
293T Cells (1)
E. coli (5)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Purified (4)
Low endotoxin level (2)
UBE2D1 / UBCH5 Protein - Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of Recombinant Oryza sativa subsp. japonica Ubiquitin-conjugating enzyme E2 5A(UBC5A) could indicate that this peptide derived from E.coli-expressed Oryza sativa subsp. japonica (Rice) UBC5A.
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E. coli
10His, N-terminus + Myc, C-terminus
23.6 kDa
1 mg/$1,679; 100 µg/$598; 20 µg/$405
Select
E. coli
GST, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
42.9 kDa
10 µg/$229; 50 µg/$274
UBE2D1 / UBCH5 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
UBE2D1 / UBCH5 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
16.4 kDa
100 µg/$494
UBE2D1 / UBCH5 Protein - 12.5% SDS-PAGE of human UBE2D1 stained with Coomassie Blue
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Wheat Germ Extract
GST
43 kDa
10 µg/$479; 25 µg/$670
UBE2D1 / UBCH5 Protein - Purified recombinant protein UBE2D1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
16.4 kDa
20 µg/$1,107
Select
E. coli
His
18.3 kDa
100 µg/$661
UBE2D1 / UBCH5 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
16.4 kDa
20 µg/$150
UBE2D1 / UBCH5 Protein
Select
E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
Select
E. coli
GST, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
42.9kD
10 µg/$229; 50 µg/$273; 500 µg/$468; 1 mg/$603
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).