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Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity.
Gene Name: | topoisomerase (DNA) III alpha |
Family/Subfamily: | Topoisomerase |
Synonyms: | TOP3A, Dna topoisomerase III, DNA topoisomerase III alpha, Topo III-alpha, TOP3, Topoisomerase (DNA) III alpha, DNA topoisomerase 3-alpha |
Target Sequences: | NM_004618 NP_004609.1 Q13472 |
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