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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Unfortunately, the antibody (ID:18850) is no longer available.

Below is a list of antibodies to the same protein target. You may also try your search again using the search box at the top of the page. Please contact us if you have any questions.



TEK / TIE2

TEK tyrosine kinase, endothelial

Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1; SHC1 and TIE1.

Gene Name: TEK tyrosine kinase, endothelial
Family/Subfamily: Protein Kinase , Tie/Tek
Synonyms: TEK, Angiopoietin-1 receptor, CD202b antigen, CD202B, Endothelial tyrosine kinase, HTIE2, Hyk, Soluble TIE2 variant 1, Soluble TIE2 variant 2, TIE-2, TIE2, VMCM, Tek tyrosine kinase, VMCM1, p140 TEK
Target Sequences: NM_000459 NP_000450.2 Q02763

Publications (2)

1
VEGFR1-positive macrophages facilitate liver repair and sinusoidal reconstruction after hepatic ischemia/reperfusion injury. Ohkubo H, Ito Y, Minamino T, Eshima K, Kojo K, Okizaki S, Hirata M, Shibuya M, Watanabe M, Majima M. PloS one. 2014 9:e105533. (WB, Flo; Mouse) [Full Text Article] [PubMed:25162491] [PMC:PMC4146544]
2
Vasculogenic and hematopoietic cellular progenitors are scattered within the prenatal mouse heart. Jankowska-Steifer E, Madej M, Niderla-Bielinska J, Ruminski S, Flaht-Zabost A, Czarnowska E, Gula G, Radomska-Lesniewska DM, Ratajska A. Histochemistry and cell biology. 2015 143:153-69. (WB; Mouse) [Full Text Article] [PubMed:25201347] [PMC:PMC4298664]

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tek-tie2 (100)
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aa1040-1120 (1)
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aa930-1010 (1)
pTyr1100 (1)
Publications
No (100)
Showing 1-25 of 100 results

TEK / TIE2 Antibody IHC-plus™ LS-B14509
Rabbit Polyclonal to Human TEK / TIE2
Rat, Human
IF IHC IHC-P WB
Unconjugated
50µl  $425.00
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

TEK / TIE2 Antibody (phospho-Tyr1100) LS-C39694
Rabbit Polyclonal (IgG) to Human TEK / TIE2
Human
IHC IHC-Fr IHC-P WB
Unconjugated
100µg  $650.00

TEK / TIE2 Antibody (clone TEK4) LS-C107063
Rat Monoclonal [clone TEK4] (IgG1,k) to Mouse TEK / TIE2
Mouse, Human
Flo IHC IHC-Fr
Unconjugated
50µg  $225.00; 100µg 0.5mg/ml  $245.00; 500µg  $455.00

TEK / TIE2 Antibody (clone 3A5) LS-C139081
Mouse Monoclonal [clone 3A5] (IgG2a,k) to Human TEK / TIE2
Mouse, Rat, Human
ELISA WB
Unconjugated
100µg 0.4mg/ml  $355.00
TEK monoclonal antibody (M20), clone 3A5. Western blot of TEK expression in PC-12.
TEK monoclonal antibody (M20), clone 3A5. Western blot of TEK expression in Raw 264.7.
TEK monoclonal antibody (M20), clone 3A5. Western blot of TEK expression in PC-12.
TEK monoclonal antibody (M20), clone 3A5. Western blot of TEK expression in Raw 264.7.

TEK / TIE2 Antibody (clone TEK4, PE) LS-C107139
Rat Monoclonal [clone TEK4] (IgG1,k) to Mouse TEK / TIE2
Mouse
Flo
PE Conjugated
50µg 0.2mg/ml  $255.00; 100µg 0.2mg/ml  $335.00; 200µg  $385.00
Two-color surface staining of mouse bone marrow with 1 ug PE anti-mouse CD202b (TEK4) and APC Sca-1 (D7) (LS-C107045) + APC CD117/c-Kit (2B8) (LS-C107024). Total viable lineage negative cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Two-color surface staining of mouse bone marrow with 1 ug PE anti-mouse CD202b (TEK4) and APC Sca-1 (D7) (LS-C107045) + APC CD117/c-Kit (2B8) (LS-C107024). Total viable lineage negative cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

TEK / TIE2 Antibody IHC-plus™ LS-B14782
Rabbit Polyclonal (IgG) to Human TEK / TIE2
Mouse, Rat, Human
IF IHC IHC-P WB
Unconjugated
50µl  $425.00
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of MCF7 cell using TEK antibody. Blue: DAPI for nuclear staining.
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of MCF7 cell using TEK antibody. Blue: DAPI for nuclear staining.

TEK / TIE2 Antibody (aa758-789) LS-C99139
Rabbit Polyclonal to Human TEK / TIE2
Human
IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human testis tissue reacted with TEK Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human testis tissue reacted with TEK Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

TEK / TIE2 Antibody LS-C39696
Rabbit Polyclonal (IgG) to Human TEK / TIE2
Human
ELISA WB
Unconjugated
50µg 1mg/ml  $445.00; 100µg  $755.00

TEK / TIE2 Antibody (clone TEK4, Biotin) LS-C107170
Rat Monoclonal [clone TEK4] (IgG1,k) to Mouse TEK / TIE2
Mouse
Flo
Biotin Conjugated
50µg  $245.00; 100µg 0.5mg/ml  $305.00; 500µg  $485.00

TEK / TIE2 Antibody (phospho-Tyr992) LS-C357288
Rabbit Polyclonal to Human TEK / TIE2
Mouse, Rat, Human
WB
Unconjugated
100µl  $305.00
Western blot of extracts from HepG2, using TIE2 (Phospho-Tyr992) Antibody.
Western blot of extracts from HepG2, using TIE2 (Phospho-Tyr992) Antibody.

TEK / TIE2 Antibody LS-C489459
Rabbit Polyclonal to Human TEK / TIE2
Human
ELISA Flo ICC IF
Unconjugated
50µl  $275.00; 100µl  $355.00

TEK / TIE2 Antibody LS-C489457
Mouse Monoclonal to Human TEK / TIE2
Human
Flo ICC IF
Unconjugated
50µl  $255.00; 100µl  $305.00; 200µl  $355.00

TEK / TIE2 Antibody LS-C746753
Rabbit Polyclonal (IgG) to Human TEK / TIE2
Mouse, Rat, Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Immunohistochemistry of paraffin-embedded human lung cancer using TEK antibody at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using TEK antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Immunohistochemistry of paraffin-embedded human lung cancer using TEK antibody at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using TEK antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

TEK / TIE2 Antibody (clone TEK4) LS-C746127
Rat Monoclonal [clone TEK4] (IgG1) to Mouse TEK / TIE2
Mouse
Flo IF
Unconjugated
25µg  $195.00

TEK / TIE2 Antibody (phospho-Tyr1108) LS-C381127
Rabbit Polyclonal (IgG) to Human TEK / TIE2
Mouse, Human
ELISA IF WB
Unconjugated
100µl 1mg/ml  $265.00

TEK / TIE2 Antibody (aa758-789) LS-C328896
Rabbit Polyclonal (IgG) to Human TEK / TIE2
Human
ELISA IHC WB
Unconjugated
200µl  $530.00

TEK / TIE2 Antibody (aa758-789, HRP) LS-C549892
Rabbit Polyclonal (IgG) to Human TEK / TIE2
Human
ELISA IHC WB
HRP Conjugated
200µl  $680.00

TEK / TIE2 Antibody (aa758-789, Biotin) LS-C529806
Rabbit Polyclonal (IgG) to Human TEK / TIE2
Human
ELISA IHC WB
Biotin Conjugated
200µl  $680.00

TEK / TIE2 Antibody (aa758-789, FITC) LS-C283604
Rabbit Polyclonal (IgG) to Human TEK / TIE2
Human
ELISA IHC WB
FITC Conjugated
200µl  $680.00

TEK / TIE2 Antibody (aa758-789, PE) LS-C283605
Rabbit Polyclonal (IgG) to Human TEK / TIE2
Human
ELISA IHC WB
PE Conjugated
200µl  $680.00

TEK / TIE2 Antibody (aa758-789, APC) LS-C283603
Rabbit Polyclonal (IgG) to Human TEK / TIE2
Human
ELISA IHC WB
APC Conjugated
200µl  $680.00

TEK / TIE2 Antibody (aa758-789, AP) LS-C511703
Rabbit Polyclonal (IgG) to Human TEK / TIE2
Human
ELISA IHC WB
AP Conjugated
200µl  $680.00

TEK / TIE2 Antibody (aa1040-1120) LS-C386425
Rabbit Polyclonal (IgG) to Human TEK / TIE2
Mouse, Human
ELISA IHC IHC-P
Unconjugated
100µl 1mg/ml  $265.00

TEK / TIE2 Antibody (phospho-Tyr992) LS-C152798
Rabbit Polyclonal (IgG) to Human TEK / TIE2
Human
IHC IP WB
Unconjugated
100µg  $405.00
WB: Untreated and pervanadate-treated HUVEC (lanes 1 and 2, respectively) were resolved by SDS-PAGE, transferred to PVDF (Immobilon-P), and probed with anti-phospho-TIE2 (Tyr992) (1:1,000). Blocking Peptide Analysis: Pervanadate-treated HUVEC lysate was either unblocked, TIE2 Tyr992 phosphorylated-peptide blocked, or TIE2 non-phosphorylated peptide blocked (lanes 3, 4, and 5, respectively) that are that were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-TIE2 (Tyr992) (1:1,000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated TIE2.
WB: Untreated and pervanadate-treated HUVEC (lanes 1 and 2, respectively) were resolved by SDS-PAGE, transferred to PVDF (Immobilon-P), and probed with anti-phospho-TIE2 (Tyr992) (1:1,000). Blocking Peptide Analysis: Pervanadate-treated HUVEC lysate was either unblocked, TIE2 Tyr992 phosphorylated-peptide blocked, or TIE2 non-phosphorylated peptide blocked (lanes 3, 4, and 5, respectively) that are that were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-TIE2 (Tyr992) (1:1,000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated TIE2.

TEK / TIE2 Antibody (phospho-Tyr1108) LS-C199653
Rabbit Polyclonal (IgG) to Human TEK / TIE2
Mouse, Human
IF Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunofluorescence analysis of HepG2 cells, using TIE2 (Phospho-Tyr1108) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from NIH/3T3 cells, using TIE2 (Phospho-Tyr1108) Antibody. The lane on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HepG2 cells, using TIE2 (Phospho-Tyr1108) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from NIH/3T3 cells, using TIE2 (Phospho-Tyr1108) Antibody. The lane on the right is blocked with the phospho peptide.
Showing 1-25 of 100 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).


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