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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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T / Brachyury

T, brachyury homolog (mouse)

Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.

Gene Name: T, brachyury homolog (mouse)
Synonyms: T / Brachyury, Brachyury, Brachyury protein, Protein T, TFT, T, T brachyury (mouse) homolog, T brachyury homolog, T, brachyury homolog (mouse)
Target Sequences: NM_003181 NP_003172.1 O15178

Filtered By: None
Target
T / Brachyury (35)
Reactivity
Human (34)
Mouse (8)
Rat (8)
Bovine (8)
Chicken (4)
Chimpanzee (6)
Dog (8)
Gibbon (6)
Guinea pig (4)
Horse (8)
Monkey (5)
Orangutan (3)
Pig (8)
Xenopus (4)
Zebrafish (4)
Application
IHC (5)
IHC-P (5)
WB (33)
Flo (2)
ELISA (12)
ICC (1)
IF (2)
RNAi (1)
Host
rabbit (21)
mouse (13)
goat (1)
Isotype
IgG (10)
IgG1 (2)
IgG1,k (7)
IgG2a,k (1)
IgM (2)
Clonality
monoclonal mc (12)
polyclonal pc (23)
Clone
1H9A2 (1)
5H8 (1)
6C12 (1)
Format
AP Conjugated (2)
APC Conjugated (2)
Azide-free (1)
Biotin Conjugated (4)
FITC Conjugated (4)
HRP Conjugated (4)
NL557 Conjugated (1)
PE Conjugated (2)
Unconjugated (16)
Epitope
aa15-43 (8)
N-Terminus (4)
aa251-300 (4)
aa 257-309 (1)
aa2-202 (1)
aa200-410 (1)
aa201-228 (1)
aa224-435 (1)
aa255-272 (1)
aa262-291 (1)
aa350-400 (1)
aa408-442 (1)
Publications
No (35)
Showing 1-25 of 35 results

T / Brachyury Antibody (aa251-300) IHC-plus™ LS-B11461
Rabbit Polyclonal to Human T / Brachyury
Dog, Bovine, Rat, Pig, Horse, Human
IHC IHC-P WB
Unconjugated
50µg  $485.00
Anti-T / Brachyury antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
T / Brachyury antibody Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-T / Brachyury antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
T / Brachyury antibody Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

T / Brachyury Antibody (aa251-300, HRP) LS-C429474
Rabbit Polyclonal to Human T / Brachyury
Dog, Bovine, Rat, Pig, Horse, Gibbon, Chimpanzee, Human
IHC IHC-P WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
Anti-T / Brachyury antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
T / Brachyury antibody Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-T / Brachyury antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
T / Brachyury antibody Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

T / Brachyury Antibody (aa251-300, Biotin) LS-C429472
Rabbit Polyclonal to Human T / Brachyury
Dog, Bovine, Rat, Pig, Horse, Gibbon, Chimpanzee, Human
IHC IHC-P WB
Biotin Conjugated
100µl 1mg/ml  $385.00
Anti-T / Brachyury antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
T / Brachyury antibody Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-T / Brachyury antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
T / Brachyury antibody Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

T / Brachyury Antibody (aa251-300, FITC) LS-C429473
Rabbit Polyclonal to Human T / Brachyury
Dog, Bovine, Rat, Pig, Horse, Gibbon, Chimpanzee, Human
IHC IHC-P WB
FITC Conjugated
100µl 1mg/ml  $385.00
Anti-T / Brachyury antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
T / Brachyury antibody Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-T / Brachyury antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
T / Brachyury antibody Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

T / Brachyury Antibody (aa 257-309, clone 1H9A2) LS-C169142
Mouse Monoclonal [clone 1H9A2] (IgG1) to Human T / Brachyury
Human
ELISA Flo IHC IHC-P WB
Unconjugated
100µg 1mg/ml  $365.00
IHC of paraffin-embedded cervical cancer tissues using T mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded rectum cancer tissues using T mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded cervical cancer tissues using T mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded rectum cancer tissues using T mouse monoclonal antibody with DAB staining.

T / Brachyury Antibody (aa350-400) LS-C148663
Rabbit Polyclonal to Human T / Brachyury
Human, Monkey
WB
Unconjugated
100µg 0.5mg/ml  $335.00
Western blot of Brachyury in U87 cell lysate. Lane 1 shows pre-immune sera, lane 2 shows Polyclonal Antibody to Brachyury tested at 2 ug/ml.
Western blot of Brachyury in U87 cell lysate. Lane 1 shows pre-immune sera, lane 2 shows Polyclonal Antibody to Brachyury tested at 2 ug/ml.

T / Brachyury Antibody (aa15-43) LS-C325542
Rabbit Polyclonal (IgG) to Human T / Brachyury
Human
ELISA WB
Unconjugated
200µl  $530.00

T / Brachyury Antibody (aa15-43, Biotin) LS-C270881
Rabbit Polyclonal (IgG) to Human T / Brachyury
Human
ELISA WB
Biotin Conjugated
200µl  $680.00

T / Brachyury Antibody (aa15-43, FITC) LS-C270882
Rabbit Polyclonal (IgG) to Human T / Brachyury
Human
ELISA WB
FITC Conjugated
200µl  $680.00

T / Brachyury Antibody (aa15-43, APC) LS-C270880
Rabbit Polyclonal (IgG) to Human T / Brachyury
Human
ELISA WB
APC Conjugated
200µl 0.6mg/ml  $680.00

T / Brachyury Antibody (aa15-43, AP) LS-C270879
Rabbit Polyclonal (IgG) to Human T / Brachyury
Human
ELISA WB
AP Conjugated
200µl  $680.00

T / Brachyury Antibody (aa15-43, Azide-free, HRP) LS-C270883
Rabbit Polyclonal (IgG) to Human T / Brachyury
Human
ELISA WB
HRP Conjugated, Azide-free
200µl  $680.00

T / Brachyury Antibody (aa15-43, PE) LS-C270885
Rabbit Polyclonal (IgG) to Human T / Brachyury
Human
ELISA WB
PE Conjugated
200µl  $680.00

T / Brachyury Antibody (aa255-272) LS-C3361
Rabbit Polyclonal to Human T / Brachyury
Human
ELISA
Unconjugated
100µg 1mg/ml  $265.00

T / Brachyury Antibody (clone 6C12) LS-C169099
Mouse Monoclonal [clone 6C12] (IgG1) to Human T / Brachyury
Human
ELISA Flo WB
Unconjugated
100µg 1mg/ml  $365.00
Western blot using T monoclonal antibody against human T recombinant protein. (Expected MW is 40.3 kDa)
Western blot using T monoclonal antibody against HEK293 (1) and T (AA: 218-352)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using T monoclonal antibody against human T recombinant protein. (Expected MW is 40.3 kDa)
Western blot using T monoclonal antibody against HEK293 (1) and T (AA: 218-352)-hIgGFc transfected HEK293 (2) cell lysate.

T / Brachyury Antibody (clone 5H8) LS-C198296
Mouse Monoclonal [clone 5H8] (IgG2a,k) to Human T / Brachyury
Human
ELISA RNAi WB
Unconjugated
100µg  $355.00
Western Blot analysis of T expression in transfected 293T cell line by T monoclonal antibody (M01), clone 5H8.Lane 1: T transfected lysate(41.1 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged T is approximately 0.03 ng/ml as a capture antibody.
Western Blot analysis of T expression in transfected 293T cell line by T monoclonal antibody (M01), clone 5H8.Lane 1: T transfected lysate(41.1 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged T is approximately 0.03 ng/ml as a capture antibody.

T / Brachyury Antibody (aa15-43) LS-C156190
Rabbit Polyclonal to Human T / Brachyury
Human
IF WB
Unconjugated
400µl  $305.00
Fluorescent confocal image of HeLa cell stained with T Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with T primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). T immunoreactivity is localized to Nucleus significantly.
T Antibody western blot of MDA-MB453 cell line lysates (35 ug/lane). The T antibody detected the T protein (arrow).
Fluorescent confocal image of HeLa cell stained with T Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with T primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). T immunoreactivity is localized to Nucleus significantly.
T Antibody western blot of MDA-MB453 cell line lysates (35 ug/lane). The T antibody detected the T protein (arrow).

T / Brachyury Antibody (aa2-202, NL557) LS-C125201
Goat Polyclonal (IgG) to Human T / Brachyury
Mouse, Human
ICC IF
NL557 Conjugated
500µl  $755.00

T / Brachyury Antibody LS-C664398
Rabbit Polyclonal (IgG) to Human T / Brachyury
Human
ELISA WB
Unconjugated
100µg  $375.00

T / Brachyury Antibody (aa224-435) LS-C125200
Mouse Monoclonal (IgG1,k) to Human T / Brachyury
Human
WB
Unconjugated
100µg  $665.00

T / Brachyury Antibody (aa262-291) LS-C161662
Mouse Monoclonal (IgM) to Human T / Brachyury
Mouse, Human
WB
Unconjugated
400µl  $305.00
T Antibody western blot of mouse kidney tissue lysates (35 ug/lane). The T antibody detected the T protein (arrow).
T Antibody western blot of mouse kidney tissue lysates (35 ug/lane). The T antibody detected the T protein (arrow).

T / Brachyury Antibody (aa201-228) LS-C161663
Mouse Monoclonal (IgM) to Human T / Brachyury
Human
WB
Unconjugated
100µl  $305.00
T Antibody western blot of NCI-H292 cell line lysates (35 ug/lane). The T antibody detected the T protein (arrow).
T Antibody western blot of NCI-H292 cell line lysates (35 ug/lane). The T antibody detected the T protein (arrow).

T / Brachyury Antibody (aa200-410) LS-C358593
Rabbit Polyclonal to Human T / Brachyury
Human
WB
Unconjugated
200µl 0.5mg/ml  $315.00

T / Brachyury Antibody (N-Terminus) LS-C134959
Rabbit Polyclonal to Human T / Brachyury
Xenopus, Chicken, Zebrafish, Pig, Horse, Mouse, Dog, Guinea pig, Bovine, Rat, Human, Monkey
WB
Unconjugated
50µg  $365.00
T / Brachyury antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
T / Brachyury antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

T / Brachyury Antibody (aa408-442) LS-C344373
Rabbit Polyclonal to Human T / Brachyury
Mouse
WB
Unconjugated
400µl  $305.00
Western blot of lysates from mouse pancreas and mouse liver tissue lysate (from left to right) with Mouse T Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
Western blot of lysates from mouse pancreas and mouse liver tissue lysate (from left to right) with Mouse T Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
Showing 1-25 of 35 results

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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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