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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SKP2

S-phase kinase-associated protein 2, E3 ubiquitin protein ligase

SKP2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates three transcript variants encoding different isoforms.

Gene Name: S-phase kinase-associated protein 2, E3 ubiquitin protein ligase
Synonyms: SKP2, F-box/LRR-repeat protein 1, FBL1, FLB1, F-box protein Skp2, FBXL1, p45, p45skp2
Target Sequences: NM_005983 NP_005974.2 Q13309

Filtered By: None
Target
SKP2 (57)
Reactivity
Human (55)
Mouse (9)
Rat (6)
Bovine (6)
Chimpanzee (3)
Gibbon (3)
Horse (1)
Monkey (4)
Pig (6)
Application
IHC (37)
IHC-P (15)
WB (53)
Flo (2)
ELISA (23)
ICC (6)
IF (6)
IP (18)
Peptide-ELISA (1)
Host
rabbit (47)
mouse (10)
Isotype
IgG (33)
IgG1 (3)
IgG1,k (7)
Clonality
monoclonal mc (10)
polyclonal pc (47)
Clone
1G12E9 (1)
6G9D10 (1)
Format
AP Conjugated (2)
APC (1)
APC Conjugated (3)
Biotin Conjugated (7)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (6)
HRP Conjugated (5)
Magnetic beads Conjugated (1)
PE Conjugated (3)
Sepharose Conjugated (1)
Unconjugated (27)
Epitope
aa43-397 (10)
C-Terminus (5)
N-Terminus (4)
aa1-424 (4)
aa2-20 (2)
340-420 aa, C-terminal (1)
Pro399 (1)
aa1-50 (1)
aa156-185 (1)
aa221-425 (1)
aa366-415 (1)
aa50-100 (1)
Publications
No (56)
Yes (1)
Showing 1-25 of 57 results

SKP2 Antibody IHC-plus™ LS-B2487
Publications (1)
Publications (1)
Mouse Monoclonal (IgG1,k) to Human SKP2
Human
ELISA IHC IHC-P IP WB
Unconjugated
50µg 0.5mg/ml  $485.00
Anti-SKP2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SKP2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

SKP2 Antibody (aa366-415) LS-C118576
Rabbit Polyclonal (IgG) to Human SKP2
Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using SKP2/p45 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using SKP2/p45 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using SKP2/p45 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using SKP2/p45 Antibody. The picture on the right is blocked with the synthesized peptide.

SKP2 Antibody LS-C9421
Rabbit Polyclonal (IgG) to Human SKP2
Human
ELISA IHC IP WB
Unconjugated
100µg 0.25mg/ml  $605.00

SKP2 Antibody (HRP) LS-C548518
Rabbit Polyclonal (IgG) to Human SKP2
Human
ELISA IHC IP WB
HRP Conjugated
100µl  $800.00

SKP2 Antibody (Biotin) LS-C528423
Rabbit Polyclonal (IgG) to Human SKP2
Human
ELISA IHC IP WB
Biotin Conjugated
100µl  $800.00

SKP2 Antibody (FITC) LS-C538376
Rabbit Polyclonal (IgG) to Human SKP2
Human
ELISA IHC IP WB
FITC Conjugated
100µl  $800.00

SKP2 Antibody (AP) LS-C510375
Rabbit Polyclonal (IgG) to Human SKP2
Human
ELISA IHC IP WB
AP Conjugated
100µl  $800.00

SKP2 Antibody (APC) LS-C518517
Rabbit Polyclonal (IgG) to Human SKP2
Human
ELISA IHC IP WB
APC Conjugated
100µl  $800.00

SKP2 Antibody (PE) LS-C656541
Rabbit Polyclonal (IgG) to Human SKP2
Human
ELISA IHC IP WB
PE Conjugated
100µl  $800.00

SKP2 Antibody (aa221-425) IHC-plus™ LS-B3361
Rabbit Polyclonal to Human SKP2
Human
IHC IHC-P
Unconjugated
250µl 0.2mg/ml  $425.00
Anti-SKP2 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-SKP2 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-SKP2 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-SKP2 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

SKP2 Antibody (aa1-50) IHC-plus™ LS-B15069
Rabbit Polyclonal (IgG) to Human SKP2
Human
IHC IHC-P IP WB
Unconjugated
50µl 1mg/ml  $425.00
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

SKP2 Antibody (C-Terminus) LS-C352844
Rabbit Polyclonal to Human SKP2
Bovine, Pig, Human
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of SKP2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of SKP2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of SKP2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of SKP2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

SKP2 Antibody LS-C416139
Rabbit Polyclonal to Human SKP2
Human
ICC IF IHC WB
Unconjugated
100µg 1mg/ml  $305.00
Western blot of 293 cell lysate using SKP2/p45 Antibody
Western blot of 293 cell lysate using SKP2/p45 Antibody

SKP2 Antibody (340-420 aa, C-terminal) LS-C386079
Rabbit Polyclonal (IgG) to Human SKP2
Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Skp2 p45 antibody
Western blot of Skp2 p45 antibody

SKP2 Antibody (N-Terminus) LS-C122446
Rabbit Polyclonal (IgG) to Human SKP2
Mouse, Rat, Human
ICC IHC IHC-P WB
Unconjugated
100µg  $530.00

SKP2 Antibody (Pro399) LS-C176209
Rabbit Polyclonal to Human SKP2
Human
IF IHC IHC-P WB
Unconjugated
100µl  $345.00
IHC of SKP2/p45 (P399) pAb in paraffin-embedded human lung carcinoma tissue.
Western blot of SKP2/p45 (P399) pAb in extracts from 293 cells.
IHC of SKP2/p45 (P399) pAb in paraffin-embedded human lung carcinoma tissue.
Western blot of SKP2/p45 (P399) pAb in extracts from 293 cells.

SKP2 Antibody LS-C408248
Rabbit Polyclonal (IgG) to Human SKP2
Mouse, Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Immunohistochemistry of paraffin-embedded mouse liver using SKP2 antibodyat dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SKP2 antibodyat dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse liver using SKP2 antibodyat dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SKP2 antibodyat dilution of 1:100. Blue: DAPI for nuclear staining.

SKP2 Antibody (N-Terminus) LS-C171853
Rabbit Polyclonal to Human SKP2
Mouse, Rat, Human
ICC IHC IHC-P WB
Unconjugated
100µg 1mg/ml  $295.00
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
 This image was taken for the unconjugated form of this product. Other forms have not been tested.

SKP2 Antibody (aa1-424) LS-C371329
Rabbit Polyclonal (IgG) to Human SKP2
Human
ELISA IHC IHC-P WB
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot. All lanes: SKP2 antibody at 2 ug/ml Lane 1:Jurkat whole cell lysate. Lane 2: HeLa whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 48 kDa. Observed band size: 48 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot. All lanes: SKP2 antibody at 2 ug/ml Lane 1:Jurkat whole cell lysate. Lane 2: HeLa whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 48 kDa. Observed band size: 48 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

SKP2 Antibody (aa1-424, HRP) LS-C371330
Rabbit Polyclonal (IgG) to Human SKP2
Human
ELISA IHC IHC-P WB
HRP Conjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot. All lanes: SKP2 antibody at 2 ug/ml Lane 1:Jurkat whole cell lysate. Lane 2: HeLa whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 48 kDa. Observed band size: 48 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot. All lanes: SKP2 antibody at 2 ug/ml Lane 1:Jurkat whole cell lysate. Lane 2: HeLa whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 48 kDa. Observed band size: 48 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

SKP2 Antibody (aa1-424, Biotin) LS-C371335
Rabbit Polyclonal (IgG) to Human SKP2
Human
ELISA IHC IHC-P WB
Biotin Conjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot. All lanes: SKP2 antibody at 2 ug/ml Lane 1:Jurkat whole cell lysate. Lane 2: HeLa whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 48 kDa. Observed band size: 48 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot. All lanes: SKP2 antibody at 2 ug/ml Lane 1:Jurkat whole cell lysate. Lane 2: HeLa whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 48 kDa. Observed band size: 48 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

SKP2 Antibody (aa1-424, FITC) LS-C371342
Rabbit Polyclonal (IgG) to Human SKP2
Human
ELISA IHC IHC-P WB
FITC Conjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot. All lanes: SKP2 antibody at 2 ug/ml Lane 1:Jurkat whole cell lysate. Lane 2: HeLa whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 48 kDa. Observed band size: 48 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot. All lanes: SKP2 antibody at 2 ug/ml Lane 1:Jurkat whole cell lysate. Lane 2: HeLa whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 48 kDa. Observed band size: 48 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

SKP2 Antibody (clone 1G12E9) LS-C202805
Mouse Monoclonal [clone 1G12E9] (IgG1) to Human SKP2
Human
Flo IHC IHC-P
Unconjugated
100µg  $650.00

SKP2 Antibody LS-C751887
Rabbit Polyclonal (IgG) to Human SKP2
Human
ELISA IHC WB
Unconjugated
100µg  $355.00

SKP2 Antibody (aa2-20) LS-C122445
Rabbit Polyclonal (IgG) to Human SKP2
Human
IHC IHC-P WB
Unconjugated
100µg  $590.00
Showing 1-25 of 57 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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