LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us

RYK

receptor-like tyrosine kinase

RYK is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Gene Name: receptor-like tyrosine kinase
Family/Subfamily: Protein Kinase , RYK
Synonyms: RYK, D3S3195, Hydroxyaryl-protein kinase, JTK5, JTK5A protein tyrosine kinase, RYK1, Vik, Tyrosine-protein kinase RYK, Receptor-like tyrosine kinase, JTK5A, Tyrosine kinase ryk
Target Sequences: NM_002958 NP_002949.2 P34925

Publications (1)

1
The Wnt receptor Ryk is required for Wnt5a-mediated axon guidance on the contralateral side of the corpus callosum. Keeble TR, Halford MM, Seaman C, Kee N, Macheda M, Anderson RB, Stacker SA, Cooper HM. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2006 26:5840-8. [PubMed:16723543] Related Antibodies: LS-C99132.

Filtered By: None
Target
RYK (65)
Reactivity
Human (62)
Mouse (31)
Rat (12)
Bat (1)
Bovine (1)
Dog (1)
Horse (1)
Monkey (1)
Xenopus (1)
Application
IHC (35)
IHC-P (3)
WB (61)
Flo (1)
ELISA (36)
ICC (2)
IF (1)
Peptide-ELISA (2)
Host
rabbit (52)
mouse (11)
goat (1)
sheep (1)
Isotype
IgG (48)
IgG1 (1)
IgG1,k (1)
IgG2b,k (9)
Clonality
monoclonal mc (11)
polyclonal pc (54)
Clone
1671CT575.42.61 (1)
240CT2.2.4 (9)
Format
AP Conjugated (4)
APC (2)
APC Conjugated (6)
Azide-free (4)
Biotin Conjugated (9)
Cy3 Conjugated (2)
Cy7 Conjugated (2)
FITC Conjugated (9)
HRP Conjugated (7)
PE Conjugated (6)
Unconjugated (20)
Epitope
aa287-533 (10)
aa308-570 (10)
aa196-413 (9)
aa160-190 (8)
aa321-348 (8)
aa561-591 (8)
aa383-410 (1)
aa98-110 (1)
Publications
No (64)
Yes (1)
Showing 1-25 of 65 results

RYK Antibody (aa561-591) LS-C99132
Publications (1)
Publications (1)
Rabbit Polyclonal to Human RYK
Human
IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-hRYK-W576 antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate. hRYK-W576(arrow) was detected using the purified antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-hRYK-W576 antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate. hRYK-W576(arrow) was detected using the purified antibody.

RYK Antibody (aa160-190) LS-C99131
Rabbit Polyclonal to Human RYK
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RYK Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RYK Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

RYK Antibody LS-C75591
Sheep Polyclonal (IgG) to Mouse RYK
Mouse
ELISA WB
Unconjugated
100µg 100µl  $770.00

RYK Antibody LS-C755065
Rabbit Polyclonal (IgG) to Human RYK
Human
ELISA IF IHC WB
Unconjugated
100µg  $355.00

RYK Antibody LS-C681243
Rabbit Polyclonal (IgG) to Human RYK
Human
ELISA IHC IHC-P WB
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

RYK Antibody (aa160-190) LS-C324875
Rabbit Polyclonal (IgG) to Human RYK
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
200µl  $530.00

RYK Antibody (aa160-190, Biotin) LS-C268687
Rabbit Polyclonal (IgG) to Human RYK
Mouse, Rat, Human
ELISA IHC WB
Biotin Conjugated
200µl  $680.00

RYK Antibody (aa160-190, FITC) LS-C268688
Rabbit Polyclonal (IgG) to Human RYK
Mouse, Rat, Human
ELISA IHC WB
FITC Conjugated
200µl  $680.00

RYK Antibody (aa160-190, AP) LS-C268684
Rabbit Polyclonal (IgG) to Human RYK
Mouse, Rat, Human
ELISA IHC WB
AP Conjugated
200µl  $680.00

RYK Antibody (aa160-190, Azide-free, HRP) LS-C268689
Rabbit Polyclonal (IgG) to Human RYK
Mouse, Rat, Human
ELISA IHC WB
HRP Conjugated, Azide-free
200µl  $680.00

RYK Antibody (aa160-190, APC) LS-C268686
Rabbit Polyclonal (IgG) to Human RYK
Mouse, Rat, Human
ELISA IHC WB
APC Conjugated
200µl  $680.00

RYK Antibody (aa160-190, PE) LS-C268690
Rabbit Polyclonal (IgG) to Human RYK
Mouse, Rat, Human
ELISA IHC WB
PE Conjugated
200µl  $680.00

RYK Antibody (aa561-591) LS-C324876
Rabbit Polyclonal (IgG) to Human RYK
Human
ELISA IHC WB
Unconjugated
200µl  $530.00

RYK Antibody (aa561-591, Biotin) LS-C268693
Rabbit Polyclonal (IgG) to Human RYK
Human
ELISA IHC WB
Biotin Conjugated
200µl  $680.00

RYK Antibody (aa561-591, FITC) LS-C268694
Rabbit Polyclonal (IgG) to Human RYK
Human
ELISA IHC WB
FITC Conjugated
200µl  $680.00

RYK Antibody (aa561-591, AP) LS-C268691
Rabbit Polyclonal (IgG) to Human RYK
Human
ELISA IHC WB
AP Conjugated
200µl  $680.00

RYK Antibody (aa561-591, APC) LS-C268692
Rabbit Polyclonal (IgG) to Human RYK
Human
ELISA IHC WB
APC Conjugated
200µl  $680.00

RYK Antibody (aa561-591, PE) LS-C268695
Rabbit Polyclonal (IgG) to Human RYK
Human
ELISA IHC WB
PE Conjugated
200µl  $680.00

RYK Antibody (aa561-591, Azide-free, HRP) LS-C268696
Rabbit Polyclonal (IgG) to Human RYK
Human
ELISA IHC WB
HRP Conjugated, Azide-free
200µl  $680.00

RYK Antibody (clone 1671CT575.42.61) LS-C757701
Mouse Monoclonal [clone 1671CT575.42.61] (IgG1,k) to Human RYK
Human
ELISA Flo WB
Unconjugated
50µl  $215.00; 200µl  $325.00
Anti-RYK Antibody at 1:1000 dilution + human lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing A431 cells stained with RYK Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (RYK Antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OJ192088) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Anti-RYK Antibody at 1:1000 dilution + human lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing A431 cells stained with RYK Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (RYK Antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OJ192088) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

RYK Antibody (aa287-533) LS-C296210
Rabbit Polyclonal (IgG) to Mouse RYK
Mouse, Human
IHC WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $275.00; 200µl 0.5mg/ml  $315.00; 1ml 0.5mg/ml  $590.00; 10ml 0.5mg/ml  $2,370.00
Western blot of recombinant RYK.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant RYK.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RYK Antibody (aa321-348) LS-C324877
Rabbit Polyclonal (IgG) to Mouse RYK
Mouse, Human
ELISA WB
Unconjugated
200µl  $530.00

RYK Antibody LS-C411036
Rabbit Polyclonal (IgG) to Human RYK
Mouse, Rat, Human
IHC WB
Unconjugated
100µl  $295.00; 200µl  $385.00

RYK Antibody (aa196-413, clone 240CT2.2.4) LS-C319202
Mouse Monoclonal [clone 240CT2.2.4] (IgG2b,k) to Human RYK
Human
ELISA WB
Unconjugated
200µl  $530.00

RYK Antibody (aa308-570) LS-C375005
Rabbit Polyclonal (IgG) to Human RYK
Human
IHC WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $265.00; 200µl 0.5mg/ml  $315.00; 1ml 0.5mg/ml  $575.00; 10ml 0.5mg/ml  $2,295.00
Western blot of RYK antibody.
Western blot of RYK antibody.
Showing 1-25 of 65 results

Selected:

If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy