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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RPS6KA2 / RSK3

ribosomal protein S6 kinase, 90kDa, polypeptide 2

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells.

Gene Name: ribosomal protein S6 kinase, 90kDa, polypeptide 2
Family/Subfamily: Protein Kinase , RSK
Synonyms: RPS6KA2, HU-2, MAPKAPK-1c, MAPKAPK1C, p90 ribosomal s6 kinase 3, p90RSK2, Pp90RSK3, Ribosomal S6 kinase 3, RSK-3, S6K-alpha, p90-RSK3, RSK, RSK3, S6K-alpha-2, S6K-alpha2, MAPKAP kinase 1c, p90-RSK 2
Target Sequences: NM_021135 NP_066958.2 Q15349

Filtered by: None
Target
RPS6KA2 / RSK3 (33)
Reactivity
Human (25)
Mouse (20)
Rat (5)
Chicken (4)
Chimpanzee (3)
Dog (4)
Guinea pig (4)
Horse (4)
Monkey (4)
Pig (4)
Rabbit (4)
Application
IHC (10)
IHC-P (4)
WB (22)
Flo (1)
ELISA (17)
ICC (1)
IF (3)
IP (2)
Peptide-ELISA (3)
RNAi (1)
Host
rabbit (30)
mouse (3)
Isotype
IgG (24)
IgG1 (1)
IgG2a,k (1)
IgG2b,k (1)
Clonality
monoclonal mc (3)
polyclonal pc (30)
Clone
1F6 (1)
3C4C8 (1)
Format
AP Conjugated (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (1)
Unconjugated (21)
Epitope
aa191-218 (8)
C-Terminus (4)
210-290 aa, Internal (1)
Asp659 (1)
Pro709 (1)
aa1-50 (1)
aa241-290 (1)
aa25-75 (1)
aa304-353 (1)
aa415-733 (1)
pThr353 (1)
Publications
No (33)
Showing 1-25 of 33 results

RPS6KA2 / RSK3 Antibody (clone 1F6) IHC-plus™ LS-B6144
Mouse Monoclonal [clone 1F6] (IgG2a,k) to Human RPS6KA2 / RSK3
Human
ELISA IF IHC IHC-P RNAi WB
Unconjugated
50µg  $495.00
Anti-RPS6KA2 / RSK3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunofluorescence of monoclonal antibody to RPS6KA2 on HeLa cell. [antibody concentration 10 ug/ml]

RPS6KA2 / RSK3 Antibody (aa415-733) LS-C62141
Mouse Monoclonal (IgG2b,k) to Human RPS6KA2 / RSK3
Human
WB
Unconjugated
100µl 1mg/ml  $305.00
Western blot Image

RPS6KA2 / RSK3 Antibody (clone 3C4C8) LS-C669187
Mouse Monoclonal [clone 3C4C8] (IgG1) to Human RPS6KA2 / RSK3
Human
ELISA Flo IF IHC WB
Unconjugated
0.1mg  $365.00

RPS6KA2 / RSK3 Antibody (aa191-218) LS-C158247
Rabbit Polyclonal to Mouse RPS6KA2 / RSK3
Mouse
WB
Unconjugated
400µl  $325.00
Mouse Rps6ka2 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The Rps6ka2 antibody detected the Rps6ka2 protein (arrow).

RPS6KA2 / RSK3 Antibody LS-C489008
Rabbit Polyclonal to Human RPS6KA2 / RSK3
Human
ICC IF IHC IHC-P
Unconjugated
50µl  $255.00; 100µl  $305.00; 200µl  $355.00

RPS6KA2 / RSK3 Antibody LS-C671832
Rabbit Polyclonal (IgG) to Human RPS6KA2 / RSK3
Human
ELISA IHC IHC-P
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry image of paraffin-embedded human lung cancer at a dilution of 1:100
Immunofluorescence staining of Hela cells with RPS6KA2 Antibody at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

RPS6KA2 / RSK3 Antibody (210-290 aa, Internal) LS-C385950
Rabbit Polyclonal (IgG) to Human RPS6KA2 / RSK3
Mouse, Human
ELISA IHC
Unconjugated
100µl 1mg/ml  $265.00

RPS6KA2 / RSK3 Antibody (aa241-290) LS-C119057
Rabbit Polyclonal (IgG) to Human RPS6KA2 / RSK3
Mouse, Human
IHC IHC-P Peptide-ELISA
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
IHC of paraffin-embedded human thyroid gland tissue, using S6K-alpha2 Antibody. The picture on the right is treated with the synthesized peptide.

RPS6KA2 / RSK3 Antibody (aa191-218) LS-C324820
Rabbit Polyclonal (IgG) to Mouse RPS6KA2 / RSK3
Mouse
ELISA WB
Unconjugated
200µl  $590.00

RPS6KA2 / RSK3 Antibody (aa191-218, Biotin) LS-C285135
Rabbit Polyclonal (IgG) to Mouse RPS6KA2 / RSK3
Mouse
ELISA WB
Biotin Conjugated
200µl  $740.00

RPS6KA2 / RSK3 Antibody (aa191-218, FITC) LS-C285138
Rabbit Polyclonal (IgG) to Mouse RPS6KA2 / RSK3
Mouse
ELISA WB
FITC Conjugated
200µl  $740.00

RPS6KA2 / RSK3 Antibody (aa191-218, APC) LS-C285136
Rabbit Polyclonal (IgG) to Mouse RPS6KA2 / RSK3
Mouse
ELISA WB
APC Conjugated
200µl  $740.00

RPS6KA2 / RSK3 Antibody (aa191-218, AP) LS-C285137
Rabbit Polyclonal (IgG) to Mouse RPS6KA2 / RSK3
Mouse
ELISA WB
AP Conjugated
200µl  $740.00

RPS6KA2 / RSK3 Antibody (aa191-218, Azide-free, HRP) LS-C285139
Rabbit Polyclonal (IgG) to Mouse RPS6KA2 / RSK3
Mouse
ELISA WB
HRP Conjugated, Azide-free
200µl  $740.00

RPS6KA2 / RSK3 Antibody (aa191-218, PE) LS-C285140
Rabbit Polyclonal (IgG) to Mouse RPS6KA2 / RSK3
Mouse
ELISA WB
PE Conjugated
200µl  $740.00

RPS6KA2 / RSK3 Antibody LS-C403497
Rabbit Polyclonal (IgG) to Human RPS6KA2 / RSK3
Mouse, Human
ELISA IHC
Unconjugated
20µl 0.3mg/ml  $205.00; 60µl 0.3mg/ml  $235.00; 120µl 0.3mg/ml  $295.00; 200µl 0.3mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human thyroid cancer using RPS6KA2 Polyclonal Antibody at dilution of 1:20.
Immunohistochemistry of paraffin-embedded Human thyroid cancer using RPS6KA2 Polyclonal Antibody at dilution of 1:20.

RPS6KA2 / RSK3 Antibody LS-C406739
Rabbit Polyclonal (IgG) to Human RPS6KA2 / RSK3
Mouse, Human
ELISA IHC
Unconjugated
20µl 0.5mg/ml  $205.00; 60µl 0.5mg/ml  $235.00; 120µl 0.5mg/ml  $295.00; 200µl 0.5mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human colon cancer using RPS6KA2 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of paraffin-embedded Human colon cancer using RPS6KA2 Polyclonal Antibody at dilution of 1:40.

RPS6KA2 / RSK3 Antibody (phospho-Thr353) LS-C291524
Rabbit Polyclonal (IgG) to Human RPS6KA2 / RSK3
Mouse, Human
ELISA Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Western blot analysis of lysates from HeLa cells, using p-Rsk-3 (Phospho-Thr353+T356) antibody.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Rsk-3 (Phospho-Thr353+T356) Antibody

RPS6KA2 / RSK3 Antibody (aa1-50) LS-C290884
Rabbit Polyclonal (IgG) to Human RPS6KA2 / RSK3
Human
IHC IP
Unconjugated
100µl 1mg/ml  $395.00

RPS6KA2 / RSK3 Antibody (aa25-75) LS-C290885
Rabbit Polyclonal (IgG) to Human RPS6KA2 / RSK3
Human
IHC IP
Unconjugated
100µl 1mg/ml  $395.00

RPS6KA2 / RSK3 Antibody LS-C753133
Rabbit Polyclonal (IgG) to Human RPS6KA2 / RSK3
Human
ELISA WB
Unconjugated
100µg  $355.00

RPS6KA2 / RSK3 Antibody LS-C770377
Rabbit Polyclonal (IgG) to Human RPS6KA2 / RSK3
Mouse, Human
ELISA WB
Unconjugated
20µl 1mg/ml  $195.00; 60µl 1mg/ml  $235.00; 120µl 1mg/ml  $285.00; 200µl 1mg/ml  $375.00

RPS6KA2 / RSK3 Antibody LS-C756603
Rabbit Polyclonal (IgG) to Human RPS6KA2 / RSK3
Mouse, Rat, Human
ELISA WB
Unconjugated
100µg  $325.00
Western blot analysis of RSK3 using anti-RSK3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates,Lane 2: human Hela whole cell lysate,Lane 3: human PC-3 whole cell lysate,Lane 4: human A431 whole cell lysate,Lane 5: human K562 whole cell lysate,Lane 6: human PANC-1 whole cell lysate,Lane 7: rat testis tissue lysates,Lane 8: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RSK3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RSK3 at approximately 90-100KD. The expected band size for RSK3 is at 83KD.

RPS6KA2 / RSK3 Antibody (aa304-353) LS-C199990
Rabbit Polyclonal (IgG) to Human RPS6KA2 / RSK3
Mouse, Human
Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Western blot of extracts from 293 cells, using Rsk-3 antibody.

RPS6KA2 / RSK3 Antibody (Pro709) LS-C75548
Rabbit Polyclonal (IgG) to Human RPS6KA2 / RSK3
Human
WB
Unconjugated
100µl  $530.00
Showing 1-25 of 33 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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